ABE1870

Anti-AHCYL1 (IRBIT) Antibody

from chicken, purified by affinity chromatography

• Synonym(s): Putative adenosylhomocysteinase 2, AHCYL1(IRBIT), AdoHcyase 2, DC-expressed AHCY-like molecule, IP(3)Rs binding protein released with IP(3), IRBIT, S-adenosyl-L-homocysteine hydrolase 2, S-adenosylhomocysteine hydrolase-like protein 1
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: chicken
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): mouse (based on 100% sequence homology)
• technique(s): western blot: suitable
• isotype: IgY
• NCBI accession no.: NP_006612
• UniProt accession no.: O43865
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... AHCYL1(10768)

Description

General description

Putative adenosylhomocysteinase 2 (EC 3.3.1.1; UniProt O43865; also known as AdoHcyase 2, DC-expressed AHCY-like molecule, IP(3)Rs binding protein released with IP(3), IRBIT, S-adenosyl-L-homocysteine hydrolase 2, S-adenosylhomocysteine hydrolase-like protein 1) is encoded by the AHCYL1 (also known as DCAL, PPP1R78, PRO0233, XPVKONA) gene (Gene ID 10768) in human. Originally identified by its binding and antagonist activity against teh intracellular calcium channel, IP3R, IRBIT is now known to interact with multiple targets and regulate diverse physiological events in addition to blocking IP3 access to IP3R. IRBIT complexes with ribonucleotide reductase (RNR) in a dATP-dependent manner and stabilizes RNR in its inactive, dATP-bound state. IRBIT also targets CaMKIIα kinase for inhibition by blocking calmodulin binding. Other known IRBIT targets include the ion transporter NBCe1-B, the Na+/H+ exchanger NHE3, the Cl- channel CFTR, the Cl−/HCO3− exchanger Slc26a6, the Fip1 component of the cleavage and polyadenylation specificity factor (CPSF) complex. IRBIT contains an S-adenosyl-l-homocysteine hydrolase (AHCY) homology domain in and an N-terminal extension containing a serine-rich region with multiple phosphorylation sites. Long-IRBIT, but not IRBIT, has an additional N-terminal appendage, termed the LISN (Long-IRBIT specific N-terminal) domain. IRBIT is highly conserved among species, and the human and mouse orthologs have 100% protein identity.

Specificity

Expected to react with both spliced isoforms reported by UniProt (O43865-1 and O43865-2).

Immunogen

Recombinant protein corresponding to human AHCYL1(IRBIT).

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

Western Blotting Analysis: A representative lot detected Tet-inducible short hairpin RNA-mediated AHCYL1 (IRBIT) downregulation in HeLa cells (Arnaoutov, A., and Dasso, M. (2014). Science 345(6203):1512-1515).
Western Blotting Analysis: A representative lot detected immunoprecipitated AHCYL1 (IRBIT) from HeLa lysates by Western blotting (Arnaoutov, A., and Dasso, M. (2014). Science 345(6203):1512-1515).
Western Blotting Analysis: A representative lot detected the association of His-tagged human AHCYL1 (IRBIT) with human IP3R1(1-604) GST fusion protein by Western blotting analysis of the affinity pull-down obtained with Glutathione beads (Arnaoutov, A., and Dasso, M. (2014). Science 345(6203):1512-1515).

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected AHCYL1 (IRBIT) in 10 µg of HeLa cell lysate.

Target description

~70 kDa observed. 59.0 kDa (isoform 1) and 53.8 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.

Physical form

Affinity purified

Purified chicken polyclonal antibody in PBS with 0.02% Sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable