ABE1858
Anti-Bora Antibody
serum, from rabbit
Synonym(s):
Protein aurora borealis, Bora, HsBora
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100 μL
$365.00
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... BORA(79866)
General description
Protein aurora borealis (UniProt Q6PGQ7; also known as HsBora) is encoded by the BORA (also known as C13orf34) gene (Gene ID 79866) in human. Originally identified as a co-factor for Aurora A-dependent asymmetrical cell divisions, Bora is an adaptor/scaffold protein that plays an important role in mitosis by mediating Aurora A-dependent Plk1 activation, and Bora depletion abolishes both Plk1 and Cdk1 activation. Cellular Bora activity is negarively regulated by Plk1- and Cdk1-mediated phosphorylation. Cdk1 initially phosphorylates Bora on S252, which primes Bora for Plk1-catalyzed S497 and T501 phosphorylation, leading to SCFβTrCP-mediated Bora ubiquitination and subsequent proteasomal degradation. The negative feed-back regulation of Bora by the kinase it activates is important, as uncontrolled Bora overexpression perturbs Aurora A and Plk1 localization, leading to lengthened metaphase. Similar to the counter regulation between Bora and Cdk1/Plk1 in somatic cells, Bora degradation is reported to be regulated via phosphorylation by Cdk1 and Plx1 in xenopus ooctyes. Cdk1 is reported to phosphorylate Bora on Thr52, which blocks its degradation. Fertilization induced calcium surge jump-starts the oscillations of the embryonic cycles by inducing Calcineurin-catalyzed Bora pThr52 dephosphorylation, leading to Bora degradation.
Specificity
This rabbit polyclonal antiserum reacts with unmodified as well as postranslationally modified (ubiquitination and phosphorylation) Bora (Qin, B., et al. (2013). J. Biol. Chem. 288(22):16139-16144). Expected to react with both spliced isoforms of human Bora reported by UniProt (Q6PGQ7).
Immunogen
Epitope: N-terminal.
GST-tagged recombinant human Bora N-terminal fragment.
Application
Anti-Bora Antibody is an antibody against Bora for use in Western Blotting, Immunoprecipitation.
Western Blotting Analysis: A representative lot detected both the endogenous Bora and a FLAG-tagged Bora exogenously expressed in HEK293T cells. Downregulation of both the endogenous and FLAG-tagged Bora was seen following replication stress induction by camptothecin (Cat. No. 208925) or UV treatment of HEK293T cells (Qin, B., et al. (2013). J. Biol. Chem. 288(22):16139-16144).
Western Blotting Analysis: A representative lot detected FLAG-tagged wild-type and T501A Bora exogenously expressed in U2OS transfectants. Only the wild-type, but not the T501A mutant, was seen downregulated upon UV irradiation of the transfected U2OS cells (Qin, B., et al. (2013). J. Biol. Chem. 288(22):16139-16144).
Immunoprecipitation Analysis: A representative lot immunorecipitated significantly higher amount of ubiquitinated Bora from UV-irradiated HEK293T cells pre-treated with the proteasome inhibitor MG-132 than UV-treated cells without MG-132 (Qin, B., et al. (2013). J. Biol. Chem. 288(22):16139-16144).
Immunoprecipitation Analysis: A representative lot immunorecipitated Bora with enhanced Thr501 phosphorylation from UV irradiated HEK293T cells (Qin, B., et al. (2013). J. Biol. Chem. 288(22):16139-16144).
Western Blotting Analysis: A representative lot detected FLAG-tagged wild-type and T501A Bora exogenously expressed in U2OS transfectants. Only the wild-type, but not the T501A mutant, was seen downregulated upon UV irradiation of the transfected U2OS cells (Qin, B., et al. (2013). J. Biol. Chem. 288(22):16139-16144).
Immunoprecipitation Analysis: A representative lot immunorecipitated significantly higher amount of ubiquitinated Bora from UV-irradiated HEK293T cells pre-treated with the proteasome inhibitor MG-132 than UV-treated cells without MG-132 (Qin, B., et al. (2013). J. Biol. Chem. 288(22):16139-16144).
Immunoprecipitation Analysis: A representative lot immunorecipitated Bora with enhanced Thr501 phosphorylation from UV irradiated HEK293T cells (Qin, B., et al. (2013). J. Biol. Chem. 288(22):16139-16144).
Quality
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: An 1:500 dilution of this antibody detected Bora in 10 µg of HEK293 cell lysate.
Western Blotting Analysis: An 1:500 dilution of this antibody detected Bora in 10 µg of HEK293 cell lysate.
Target description
~58 kDa observed. 61.20 kDa (isoform 1) and 53.44 kDa (isoform 2) calculated.
Other Notes
Concentration: Please refer to lot specific datasheet.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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