ABE1817
Anti-FANCI Antibody, (G4270)
from rabbit, purified by affinity chromatography
Synonym(s):
Fanconi anemia group I protein, Protein FACI
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100 μG
$414.00
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... FANCI(55215)
General description
Fanconi anemia group I protein (UniProt Q9NVI1; also known as Protein FACI) is encoded by the FANCI (also known as KIAA1794) gene (Gene ID 55215) in human. It plays a vital role in the repair of DNA double-strand breaks by homologous recombination and in the repair of interstrand DNA cross-links (ICLs) by promoting FANCD2 monoubiquitination by FANCL and participating in recruitment to DNA repair sites. It is also required for maintenance of chromosomal stability. FACI is reported to participate in S phase and G2 phase checkpoint activation upon DNA damage. It is monoubiquitinated by FANCL on Lys-523 during S phase and upon genotoxic stress and is deubiquitinated by USP1 as cells enter G2/M or once the DNA repair is completed. Mutations in the FANCI gene are known to cause Fanconi anemia, a developmental and cancer-predisposition syndrome. Ref: Smogorzewska A., et al. (2007) Cell 129, 289-301.
Specificity
This polyclonal antibody detected the expected target band only in lysate from FANCI-tranfected, but not untransfected FANCI-deficient EUFA1301 cells.
Immunogen
Synthetic peptide corresponding to an internal sequence within the N-terminal half of human FANCI.
Application
Anti-FANCI (G4270), Cat. No. ABE1817, is a highly specific rabbit polyclonal antibody that targets FANCI and has been tested in Western Blotting.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Western Blotting Analysis: 1.4 µg/mL from a representative lot detected exogenously expressed FANCI in transfected, but not untransfected FANCI-deficient EUFA1301 cells (kindly provided by Dr. Johan de Winter) with or without 24-hour 60 ng/ml mitomycin C (MMC) treatment (Courtesy of Martin Cohn, Ph.D., Department of Biochemistry, University of Oxford, UK).
Western Blotting Analysis: 1.4 µg/mL from a representative lot detected endogenous FANCI in FANCD2-deficient EUFA1421 (kindly provided by Dr. Johan de Winter) cells. Exogenous FANCD2 expression stabilized FANCI and monoubiquitinated FANCI was detectable upoon 24-hour 60 ng/ml mitomycin C (MMC) treatment of FANCD2 transfectants (Courtesy of Martin Cohn, Ph.D., Department of Biochemistry, University of Oxford, UK).
Western Blotting Analysis: 1.4 µg/mL from a representative lot detected monoubiquitinated FANCI mainly in the nuclear fraction of FANCD2-deficient EUFA1421 cells (kindly provided by Dr. Johan de Winter) upon exogenous FANCD2 expression with or without additional 24-hour 60 ng/ml mitomycin C (MMC) treatment (Courtesy of Martin Cohn, Ph.D., Department of Biochemistry, University of Oxford, UK).
Western Blotting Analysis: 1.4 µg/mL from a representative lot detected endogenous FANCI in FANCD2-deficient EUFA1421 (kindly provided by Dr. Johan de Winter) cells. Exogenous FANCD2 expression stabilized FANCI and monoubiquitinated FANCI was detectable upoon 24-hour 60 ng/ml mitomycin C (MMC) treatment of FANCD2 transfectants (Courtesy of Martin Cohn, Ph.D., Department of Biochemistry, University of Oxford, UK).
Western Blotting Analysis: 1.4 µg/mL from a representative lot detected monoubiquitinated FANCI mainly in the nuclear fraction of FANCD2-deficient EUFA1421 cells (kindly provided by Dr. Johan de Winter) upon exogenous FANCD2 expression with or without additional 24-hour 60 ng/ml mitomycin C (MMC) treatment (Courtesy of Martin Cohn, Ph.D., Department of Biochemistry, University of Oxford, UK).
Quality
Evaluated by Western Blotting in K562 nuclear extract.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected FANCI in 10 µg of K562 nuclear extract.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected FANCI in 10 µg of K562 nuclear extract.
Target description
~160 kDa observed. 149.3 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Affinity purified.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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David Lopez-Martinez et al.
Cell reports, 27(10), 2990-3005 (2019-06-06)
Interstrand crosslinks (ICLs) of the DNA helix are a deleterious form of DNA damage. ICLs can be repaired by the Fanconi anemia pathway. At the center of the pathway is the FANCD2/FANCI complex, recruitment of which to DNA is a
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