ABE1426
Anti-COUP-TF2 Antibody
serum, from rabbit
Synonym(s):
COUP transcription factor 2, COUP-TF2, Apolipoprotein AI regulatory protein 1, ARP-1, COUP transcription factor II, COUP-TF II, Nuclear receptor subfamily 2 group F member 2, COUP-TF2
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
biological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, mouse
technique(s)
ChIP: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Gene Information
human ... NR2F2(7026)
mouse ... Nr2F2(11819)
General description
COUP transcription factor 2 (UniProt P43135; also known as Apolipoprotein AI regulatory protein 1, Apolipoprotein regulatory protein 1, ARP-1, COUP-TF II, COUP transcription factor II, Nuclear receptor subfamily 2 group F member 2) is encoded by the Nr2f2 (also known as Aporp1, Arp-1, Arp1, Tfcoup2) gene (Gene ID 11819) in murine species. COUP transcription factors1&2 (COUP-TFI & COUP-TFII) are two related nuclear orphan receptors implicated in their roles in guiding migrating neurons towards their target regions during brain development. COUP-TFI and COUP-TFII are expressed in distinct and non-overlapping migrating cells in specific pathways in the basal telencephalon of mouse embryos at a stage when interneuron migration is robust. While COUP-TFI appears to control dorsal and ventral migration in the basal telencephalon, COUP-TFII is more specific in modulating tangential migration into the intermediate zone of the cortex. Coup-TFII is also identified as an activator of Prox1 expression in venous lymphatic endothelial cell (LEC) progenitors during early stages of LEC specification and differentiation.
Specificity
Reacts with COUP-TF2, but not COUP-TF1.
Immunogen
Epitope: N-terminal half
GST-tagged recombinant protein corresponding to the the N-terminal half of mouse COUP-TF2.
Application
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected COUP-TF2 in mouse cerebral cortex.
Immunofluorescence Analysis: A 1:500-1000 dilution of a representative lot detected COUP-TF2 immunoreactivity in the developing brain using paraformaldehyde-fixed cryosections from wild-type mouse E13.5 embryos, but not from embryos with conditional brain region COUP-TF2-knockout, by fluorescent immunohistochemistry (Courtesy of Dr. Michele Studer, Institut de Biologie Valrose, France).
Immunohistochemistry Analysis: A representative lot detected a different COUP-TF2 expression pattern than that of COUP-TF1 in the developing telencephalon of forebrain using cryosections from E13.5 mouse embryos (Tripodi, M., et al. (2004). Development. 131(24):6119-6129).
Immunocytochemistry Analysis: A representative lot detected COUP-TF2I immunoreactivity in reelin-positive Cajal-Retzius cells, but not in calbindin-positive GABAergic interneurons in the developing cortex by dual fluorescent immunohistochemistry using cryosections from E13.5 and E15.5 mouse embryos (Tripodi, M., et al. (2004). Development. 131(24):6119-6129).
Chromatin Immunoprecipitation Analysis: A representative lot detected COUP-TF2 occupancy at the conserved COUP-TFII binding motif in the regulatory region of Prox1 using chromatin preparations from human lymphatic endothelial cells (LECs) (Srinivasan, R.S., et al. (2010). Genes Dev. 24(7):696-707).
Western Blotting Analysis: A representative lot detected COUP-TF2 expression in HEK293T and NIH3T3 cells (Srinivasan, R.S., et al. (2010). Genes Dev. 24(7):696-707).
Immunofluorescence Analysis: A 1:500-1000 dilution of a representative lot detected COUP-TF2 immunoreactivity in the developing brain using paraformaldehyde-fixed cryosections from wild-type mouse E13.5 embryos, but not from embryos with conditional brain region COUP-TF2-knockout, by fluorescent immunohistochemistry (Courtesy of Dr. Michele Studer, Institut de Biologie Valrose, France).
Immunohistochemistry Analysis: A representative lot detected a different COUP-TF2 expression pattern than that of COUP-TF1 in the developing telencephalon of forebrain using cryosections from E13.5 mouse embryos (Tripodi, M., et al. (2004). Development. 131(24):6119-6129).
Immunocytochemistry Analysis: A representative lot detected COUP-TF2I immunoreactivity in reelin-positive Cajal-Retzius cells, but not in calbindin-positive GABAergic interneurons in the developing cortex by dual fluorescent immunohistochemistry using cryosections from E13.5 and E15.5 mouse embryos (Tripodi, M., et al. (2004). Development. 131(24):6119-6129).
Chromatin Immunoprecipitation Analysis: A representative lot detected COUP-TF2 occupancy at the conserved COUP-TFII binding motif in the regulatory region of Prox1 using chromatin preparations from human lymphatic endothelial cells (LECs) (Srinivasan, R.S., et al. (2010). Genes Dev. 24(7):696-707).
Western Blotting Analysis: A representative lot detected COUP-TF2 expression in HEK293T and NIH3T3 cells (Srinivasan, R.S., et al. (2010). Genes Dev. 24(7):696-707).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
Nuclear Receptors
This Anti-COUP-TF2 Antibody is validated for use in Immunohistochemistry (Paraffin), Immunofluorescence, Chromatin Immunoprecipitation (ChIP), Western Blotting for the detection of COUP-TF2.
Quality
Evaluated by Immunohistochemistry in mouse hippocampus tissue.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected COUP-TF2 in mouse hippocampus tissue.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected COUP-TF2 in mouse hippocampus tissue.
Target description
45 kDa calculated
Physical form
Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Max Anstötz et al.
eNeuro, 7(1) (2020-01-08)
The study of brain circuits depends on a clear understanding of the role played by different neuronal populations. Therefore, the unambiguous identification of different cell types is essential for the correct interpretation of experimental data. Here, we emphasize to the
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
