ABE1403

Anti-Cdk1/cdc2 Antibody C-term

from rabbit, purified by affinity chromatography

• Synonym(s): Cyclin-dependent kinase 1, CDK1, Cell division control protein 2 homolog, Cell division protein kinase 1, p34 protein kinase
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, mouse
• species reactivity (predicted by homology): rat (based on 100% sequence homology), hamster (based on 100% sequence homology)
• technique(s): immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI accession no.: NP_001777
• UniProt accession no.: P06493
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: hamster ... Cdk1(100766716) ; human ... CDK1(983) ; mouse ... Cdk1(12534) ; rat ... Cdk1(54237)

Description

General description

Cyclin-dependent kinase 1 (Cdk1) is also known as Cell division control protein 2 homolog (Cdc2. ;Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. Activation of cdc2 is controlled at several steps including cyclin binding and phosphorylation of Thr161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14. Phosphorylation at Tyr15 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase. Expression of cdc2 plays a critical role in cell transition through the G2/M phase. The activity of cdc2 in dividing cells increases at the onset of M phase coincident with disassembly of the cell nucleus, generation of mitotic spindles and chromosome condensation. Several proteins including Histone H1 and pp60src are substrates for cdc2.

Immunogen

Epitope: Near C-terminus

KLH-conjugated linear peptide corresponding to human Cdk1/cdc2 C-term near the C-terminus.

Application

Immunoprecipitation Analysis: 2.0 µg/mL from a representative lot immunoprecipitated Cdk1/cdc2 in 500 µg of Balb/3T3/A31 cell lysate.
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Cdk1/cdc2 in human testis and human breast cancer tissue.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-Cdk1/cdc2 Antibody C-term is validated for use in Western Blotting and Immunohistochemistry and Immunoprecipitation for the detection of Cdk1/cdc2 C-term.

Quality

Evaluated by Western Blotting in Balb/3T3/A31 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Cdk1/cdc2 in 10 µg of Balb/3T3/A31 cell lysate.

Target description

~34 kDa observed. Higher molecular weight band may correspond to phosphorylated form of protein.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable