ABE1042

Anti-monomethyl Histone H3 Antibody (Lys18)

from rabbit, purified by affinity chromatography

• Synonym(s): Yeast (S.cerevisiae) partial histone 3 (H3) gene, H3K18me1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, yeast
• technique(s): ChIP: suitable; dot blot: suitable; western blot: suitable
• GenBank accession no.: AAA34659
• shipped in: wet ice
• target post-translational modification: monomethylation (Lys18)
• Gene Information: human ... H3C1(8350)

Description

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Immunogen

Linear peptide corresponding to yeast Histone H3 monomethylated at Lys18.

Application

Detect methylated Histone H3 using this rabbit polyclonal antibody, Anti-monomethyl Histone H3 Antibody (Lys18) validated for use in western blotting & Dot Blot.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Western Blotting Analysis: A representative lot detected monomethyl Histone H3 (Lys18) in yeast whole cell extracts, which express wild-type monomethyl Histone H3 (Lys18) and not mutant monomethyl Histone H3 (Lys18Ala) (Prof. F. Howe from the laboratory of Prof. J. Mellor, University of Oxford.).

Dot Blot Analysis: A representative lot detected monomethyl Histone H3 (Lys18) peptide and not unmodified Histone H3 (Prof. F. Howe from the laboratory of Prof. J. Mellor, University of Oxford.).

Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated monomethyl Histone H3 (Lys18) in 1.6X 10E7 yeast cell equivalents (Prof. F. Howe from the laboratory of Prof. J. Mellor, University of Oxford.).

Quality

Evaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: 1:1,000 dilution of this antibody detected monomethyl Histone H3 (Lys18) in 10 µg of HeLa acid extract.

Target description

~17 kDa observed

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Legal Information

GenBank is a registered trademark of United States Department of Health and Human Services

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable