ABE1033

Anti-RBM4 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): RNA-binding protein 4, Lark homolog, hLark, RNA-binding motif protein 4, RNA-binding motif protein 4a, RBM4
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): monkey (based on 100% sequence homology)
• technique(s): immunocytochemistry: suitable; western blot: suitable
• NCBI accession no.: NP_002887
• UniProt accession no.: Q9BWF3
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... RBM4(5936)

Description

General description

RBM4 is a widely expressed RNA-binding factor involved in many cellular process, including alternative splicing of pre-mRNA and translation regulation by modulating alternative 5′-splice site and exon selection on target hnRNA. RBM4 plays roles in development, particularly muscle cell development, clock metabolism and cellular stress. In muscle, RBM4 acts as a muscle cell differentiation-promoting factor and activates exon skipping of the PTB pre-mRNA during muscle cell differentiation, and it antagonizes the activity of the splicing factor PTBP1 to modulate muscle cell-specific exon selection of alpha tropomyosin. RBM4 also mediates various mRNA functions important for myocytes, and RBM4 associates with important miRNAs that are important for muscle cell differentiation. RBM4 is also involved in regulation of the biological clock because it is required for the translational activation of PER1 mRNA in response to circadian clock. In cellular stress, RBM4 recruits EIF4A1 to stimulate IRES-dependent translation initiation in response to cellular stress, and RBM4 associates to internal ribosome entry segment (IRES) in target mRNA species under stress conditions.

Immunogen

His-tagged recombinant protein corresponding to human RBM4.

Application

Anti-RBM4 Antibody is an antibody against RBM4 Antibody for use in Western Blotting, Immunocytochemistry.

Immunocytochemistry Analysis: 2.0 µg/mL from a representative lot detected RBM4 in HeLa and A431 cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

Quality

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:250 dilution of this antibody detected RBM4 in 10 µg of HepG2 cell lysate.

Target description

~42 kDa observed

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable