Tumor necrosis factor ligand superfamily member 18, also known as Activation-inducible TNF-related ligand (AITRL) or Glucocorticoid-induced TNF-related ligand (hGITRL) and encoded by the gene name TNFSF18 or AITRL or GITRL or TL6, regulates numerous biological functions including cell proliferation, differentiation, and survival through regulating the activation of the transcription factor NF-κB and various mitogen-activated protein kinases. The glucocorticoid-induced tumor necrosis factor receptor (GITR) is an emerging member of this family that is expressed on CD4+ CD25+ regulatory T cells and appears to have crucial immune regulation functions. Its ligand GITRL is expressed in endothelial and antigen-presenting cells (4) and can activate NF-κB, induce both pro- and anti-apoptotic effects, inhibit the suppressive activity of regulatory T cells, and co-stimulate responder T cells through GITR. Dominant negative forms of NIK and TRAF2 expressed in transfected 293 cells substantially inhibited NF-κB activation, suggesting that the GITRL-GITR pathway involves both NIK and TRAF2.
Immunogen
KLH-conjugated recombinant human GITRL ligand.
Application
Detect GITRL using this Anti-GITRL Antibody validated for use in western blotting & ICC.
Immunocytochemistry Analysis: 10 μg/mL from a representative lot detected GITRL in THP-1 cells.
Research Category Apoptosis & Cancer
Research Sub Category Apoptosis - Additional
Quality
Evaluated by Western Blotting in recombinant GITRL protein.
Western Blotting Analysis: 1 μg/mL of this antibody was detected in recombinant GITRL protein.
Target description
~26 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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