ABC460

Anti-Sec23B Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Tumor-associated calcium signal transducer 2, Cell surface glycoprotein Trop-2, Membrane component chromosome 1 surface marker 1, Pancreatic carcinoma marker protein GA733-1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse
• technique(s): western blot: suitable
• NCBI accession no.: NP_001239472
• UniProt accession no.: Q9D662
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... TACSTD2(4070)

Description

General description

The protein named Sec23B, also known as protein transport protein Sec23B, or SEC23-related protein B is a component of the COPII coat, the complex that governs ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. The transport of proteins in eukaryotic cells from the endoplasmic reticulum (ER) to the Golgi complex proceeds by deformation of specialized portions of the donor membrane to form carrier vesicles. A group of cytosolic proteins collectively known as COPII carry out a programmed set of sequential interactions, leading to cargo sorting and vesicle budding. The Sec23B protein is critical to proper protein transport in and out of cells, and it is localized to the smooth endoplasmic reticulum membrane where it is a peripheral membrane protein and interacts with the Golgi apparatus membranes as well. Sec23B is expressed at high levels in brain and fibroblasts as well as liver and other metabolically active tissues. Defects in Sec23B cause congenital dyserythropoietic anemia type II, a recessive disorder of erythroid differentiation but the disease is not caused by loss of function of Sec23B but rather a combination of a hypomorphic allele with altered functionality appears to be the cause and its related protein Sec23A can often compensate somewhat for the defect.

Immunogen

Linear peptide corresponding to mouse Sec23B.

Application

Detect Sec23B using this rabbit polyclonal antibody, Anti-Sec23B Antibody validated for use in western blotting.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Western Blotting Analysis: A representative lot from an independent laboratory detected Sec23B in various mouse tissue lysates expressing wild-type Sec23B. The same lot detected Sec23B in Sec23B wild-type MEF cell lysates and demonstrated a loss of signal in Sec23B knockdown MEF cell lysates (Tao, J., et al. (2012). Proc Natl Acad Sci USA. 109(29):E2001-E2009.).

Quality

Evaluated by Western Blotting in mouse pancreas tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Sec23B in 10 µg of mouse pancreas tissue lysate.

Target description

~86 kDa observed. Uncharacterized Band(s) may be observed in some cell lysates.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable