ABC248

Anti-HPGD Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): 15-hydroxyprostaglandin dehydrogenase [NAD(+)], Prostaglandin dehydrogenase 1, 15-PGDH
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): primate (based on 100% sequence homology), rabbit (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), baboon (based on 100% sequence homology), monkey (based on 100% sequence homology)
• technique(s): immunohistochemistry: suitable; western blot: suitable
• NCBI accession no.: NP_000851.2
• UniProt accession no.: P15428
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... HPGD(3248)

Description

General description

The protein named HPGD, or also known as 15-PGDH, or Prostaglandin dehydrogenase 1, and encoded by the gene HPGD/PGDH1, is a protein necessary for the regulation of prostaglandin levels. Prostaglandins are potent inflammatory agents, thus their regulation is key for control inflammatory responses. HPGD works to mediate prostaglandin inactivation by catalyzing the NAD dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoin A4. Additionally, HPGD inhibits in vivo proliferation of colon cancer cells. HPGD is localized to the cytoplasm. HPGD is expressed in intestinal and colon tissues. HPGD is down regulated in colon cancer and thus is being examined as a therapeutic agent. Defects in HPGD may be associated with Hypertrophic osteoarthropathy, a disease characterized by digital clubbing, periostosis, acroosteolysis, painful joint enlargement, and variable features of pachydermia that include thickened facial skin and a thickened scalp. Other developmental anomalies include delayed closure of the cranial sutures and congenital heart disease. Other diseases associated with this gene’s defects are Crainoosteoarthropathy (swollen joint disease) and congenital nail clubbing where one has very large nails on the fingers and toes.

Immunogen

KLH-conjugated linear peptide corresponding to human HPGD.

Application

Detect Prostaglandin dehydrogenase 1 using this rabbit polyclonal antibody, Anti-HPGD Antibody validated for use in western blotting & IHC.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected HPGD in human ileum tissue.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Quality

Evaluated by Western Blotting in Caco-2 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected HPGD in 10 µg of Caco-2 cell lysate.

Target description

~32 kDa observed

Physical form

Affinity purified

Purified rabbit Polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable