ABC242

Anti-CRKL Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Crk-like protein
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat, human, mouse
• species reactivity (predicted by homology): rhesus macaque (based on 100% sequence homology)
• technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• NCBI accession no.: NP_005198
• UniProt accession no.: P46109
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CRKL(1399)

Description

General description

Crk-like protein (CRKL) is a 39 kDa adaptor protein with one SH2 and two SH3 binding domains. CRKL is the major protein which is tyrosine phosphorylated in response to BCR/ABL (chronic myelogenous leukemia) and growth factor activation. In myeloid cells, it binds to proteins such as paxillin, p130cas and p120 cbl. CRKL aslo appears to bind ABL and SOS.

Immunogen

KLH-conjugated linear peptide corresponding to human CRKL.

application

Detect Crk-like protein using this rabbit polyclonal antibody, Anti-CRKL Antibody validated for use in western blotting & IP.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Western Blotting Analysis: 0.25 µg/mL from a representative lot detected CRKL in 10 µg of NIH3T3 and L6 cell lysate.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated CRKL in 500 µg of Jurkat cell lysate.

Quality

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected CRKL in 10 µg of Jurkat cell lysate.

Target description

~39 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable