AB5976

Anti-Dishevelled-2 Antibody

Chemicon^®, from rabbit

• Synonym(s): DVL2, DSH Homolog 2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 300
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): western blot: suitable
• input: sample type hematopoietic stem cell(s); sample type neural stem cell(s)
• NCBI accession no.: NM_004422.2
• UniProt accession no.: O14641
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... DVL2(1856)

Description

General description

DVL2 is a member of multigene family that also contains DVL1 and DVL3. The vertebrate dishevelled proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. DVL2 encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein.

Specificity

Dishevelled-2 protein (dvl-2). By Western blot the antibody shows no cross reactivity with recombinant dishevelled-1 (dvl-1) or dishevelled-3 (dvl-3) protein. Pre-adsorption of the antibody with the immunogen peptide completely abolishes its reactivity by Western blot against disheveiled-2 protein.

Immunogen

Synthetic peptide corresponding to amino acids 633-645 of human disheveiled-2 protein.

Application

Research Category
Stem Cell Research

Research Sub Category
Hematopoietic Stem Cells

Neural Stem Cells

This Anti-Dishevelled-2 Antibody is validated for use in WB for the detection of Dishevelled-2.

Western blot: 1:1,000 using ECL. The antibody reacts with a protein of ~95kDa.

Dilute antibody in 50 mM PBS, pH 7.4 containing 1.5% sodium chloride and 1% normal animal serum (same species as the host of the secondary antibody).

Optimal working dilutions must be determined by end user.

Physical form

Format: Purified

Partially purified immunoglobulin. Liquid in PBS, pH 7.4, containing 0.01M sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots (not less than 10 μL) for up to 6 months. Avoid repeated freeze/thaw cycles. The use of high quality antiserum-grade plastic or glass is recommended.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids