AB10423

Anti-Phospho- Bad (Ser75) Antibody

from rabbit

• Synonym(s): BCL-X/BCL-2 binding protein, BCL2-antagonist of cell death protein, BCL2-associated agonist of cell death, BCL2-binding component 6, BCL2-binding protein, Bcl-2-binding component 6, Bcl-2-like 8 protein, Bcl-XL/Bcl-2-associated death promoter
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse, rat
• species reactivity (predicted by homology): human (based on 100% sequence homology)
• technique(s): western blot: suitable
• NCBI accession no.: NP_116784
• UniProt accession no.: Q92934
• shipped in: wet ice
• target post-translational modification: phosphorylation (pSer75)
• Gene Information: human ... BAD(572)

Description

General description

Bad is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin are found to be involved in the regulation of this protein. Bad is phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation.

Specificity

Other species have not been tested.

This antibody reacts with the 18 kDa phospho-Bad (Ser75) protein.

Immunogen

This antibody is generated in rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS75 of human Bad.

Application

Anti-Phospho-Bad (Ser75) Antibody detects level of Phospho-Bad (Ser75) & has been published & validated for use in WB.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Quality

Western Blot Analysis: 1:250 dilution of this antibody detected phospho-Bad (Ser75) in lysate from LPS-stimulated RAW264.7 cells.

Target description

18 kDa

Physical form

Format: Purified

Protein G followed by IAP

Purified immunoglobulins in PBS with 0.09% NaN3.

Storage and Stability

Store at 2-8°C for 6 months from date of receipt. For long term storage, store at -20°C

Analysis Note

Control
Western Blot : RAW264.7/LPS lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable