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AB10418

Sigma-Aldrich

Anti-Puma BH3 domain Antibody

from rabbit

Synonym(s):

BCL2 binding component 3, Bcl-2 binding component 3, p53 up-regulated modulator of apoptosis

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse

technique(s)

western blot: suitable

NCBI accession no.

NP_055232

UniProt accession no.

Q9BXH1

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Bbc3(170770)

General description

PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis

Specificity

Other species have not been tested.
The antibody reacts with the 21 kDa Puma BH3 domain protein.

Immunogen

This antibody was developed against a synthetic peptide selected from the C-terminal region of human Puma.

Application

Anti-Puma BH3 domain Antibody is an antibody against Puma BH3 domain for use in WB.

Quality

Western Blot Analysis: 1:250 dilution of this antibody detected Puma BH3 domain on mouse brain lysate.

Target description

21 kDa

Physical form

Format: Purified

Analysis Note

Control
Western Blot : Mouse brain lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Bhargab Kalita et al.
PloS one, 11(12), e0168525-e0168525 (2016-12-31)
Development of an effective radio protector to minimise radiation-inflicted damages have largely failed owing to inherent toxicity of most of the agents examined so far. This study is centred towards delivering protection to lethally irradiated mice by pre-administration of a

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