681637

Wee1/Chk1 Inhibitor - CAS 1177150-89-8 - Calbiochem

The Wee1/Chk1 Inhibitor, also referenced under CAS 1177150-89-8, controls the biological activity of Wee1/Chk1. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.

• Synonym(s): Wee1/Chk1 Inhibitor - CAS 1177150-89-8 - Calbiochem, 4-(2-Phenyl)-9-hydroxypyrrolo[3,4-c]carbazole-1,3-(2H,6H)-dione, Chk1 Inhibitor IV, Wee1 Inhibitor III
• Empirical Formula (Hill Notation): C₂₀H₁₂N₂O₃ · xH₂O
• CAS Number: 1177150-89-8
• Molecular Weight: 328.32 (anhydrous basis)
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• assay: ≥95% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: orange
• solubility: DMSO: 200 mg/mL; ethanol: 25 mg/mL
• shipped in: ambient
• storage temp.: −20°C

Description

General description

A pyrrolocarbazole compound that acts as a potent and ATP-competitive inhibitor of checkpoint kinases Wee1 and Chk1 (IC₅₀ = 97 nM and 47 nM, respectively). Inhibits PKC, Cdk4, or other CDKs only at much higher concentrations (IC₅₀ = 3.4 µM, 3.75 µM, and >5 µM, respectively) and exhibits little effect against FGFR, PDGFR, or c-Src (IC₅₀ >50 µM).

A pyrrolocarbazole compound that acts as a potent and ATP-competitive inhibitor of checkpoint kinases Wee1 and Chk1 (IC₅₀ = 97 nM and 47 nM, respectively). Inhibits PKC, Cdk4, or other CDKs only at much higher concentrations (IC₅₀ = 3.4 µM, 3.75 µM, and >5 µM, respectively) and exhibits little effect against FGFR, PDGFR, or c-Src (IC₅₀ >50µM).

Biochem/physiol Actions

Cell permeable: no

Primary Target
Wee1 and Chk1

Product competes with ATP.

Reversible: no

Target IC₅₀: 97 nM and 47 nM against Wee1 and Chk1, respectively

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Palmer, B.D., et al. 2006. J. Med. Chem.49, 4896.
Squire, C.J., et al. 2005. Structure13, 541.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10-13 - German Storage Class 10 to 13