616430

TGF-α, Human, Recombinant, E. coli

• Synonym(s): TGF-α, Human, Recombinant, E. coli, Transforming Growth Factor-α
• CAS Number: 105186-99-0
• MDL number: MFCD00082077
• UNSPSC Code: 12352202

Properties

• assay: ≥97% (SDS-PAGE)
• Quality Level: 100
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• impurities: ≤100 pg/μg Endotoxin (pg/μg TGF-α)
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

Recombinant, human transforming growth factor-α expressed in E. coli. Plays an important role in cell-cell adhesion and cell growth and differentiation. Reported to play a role in the development of hormonal insensitivity in estrogen-receptor-positive breast cancers.

Biochem/physiol Actions

ED₅₀= 0.1-0.4 ng/ml as measured by the ability to stimulate ³H-thymidine incorporation in the mouse fibroblast cell line Balb/3T3

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from 30% acetonitrile, 0.1% TFA.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.

Reconstitute to a concentration of ≥10 µg/ml with sterile 10 mM acetic acid containing ≥0.1% HSA or BSA.

Other Notes

Nicholson, R.I., et al. 1994. Cancer Res. 54, 1684.
Anklesaria, P., et al. 1990. Proc. Natl. Acad. Sci. USA87, 3289.
Massague, J. 1990. J. Biol. Chem.265, 21393.
Watanabe, S., et al. 1987. Proc. Natl. Acad. Sci. USA84, 1258.
Schreiber, A.B., et al. 1986. Science232, 1250.
Tam, J.P. 1985. Science229, 673.
Derynck, R., et al. 1984. Cell38, 327.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany