616371

Aprotinin, Bovine, Recombinant, Nicotiana sp., Animal-Free

recombinant, expressed in Nicotiana, heat and acid-stable competitive, reversible inhibitor of serine proteases

• Synonym(s): Aprotinin, Bovine, Recombinant, Nicotiana sp., Animal-Free, Pancreatic Trypsin Inhibitor, Trypsin-Kallikrein Inhibitor, Kallikrein-Trypsin Inactivator, Antikrein, Basic Pancreatic Trypsin Inhibitor
• Empirical Formula (Hill Notation): C₂₈₄H₄₃₂N₈₄O₇₉S₇
• CAS Number: 9087-70-1
• Molecular Weight: 6511.44
• MDL number: MFCD00130541
• UNSPSC Code: 12352202
• EC Index Number: 2329949
• NACRES: NA.77

Properties

• recombinant: expressed in Nicotiana
• Quality Level: 200
• assay: ≥98% (SDS-PAGE)
• form: powder
• specific activity: ≥5.0 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white to off-white
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Recombinant, bovine aprotinin supplied without animal-derived components. Aprotinin is a competitive, reversible inhibitor of proteolytic and esterolytic activity. A relatively heat- and acid-stable serine protease inhibitor. Forms a tight complex, blocking the active site of the enzyme. Effective at concentrations equimolar with protease. Inhibits several proteases, including coagulation factors in the prephase of blood clotting, tissue and leukocytic proteinases, chymotrypsin, trypsin (K_d = 5 x 10^-14 M), plasmin (K_d = 2.3 x 10^-10 M), and kallikrein (K_d = 1 x 10^-7 M). Proteases not inhibited by aprotinin include Factor Xa, thrombin, pepsin, papain, and carboxypeptidases A and B. Useful for protein purification and for extending the life of cells in culture by preventing proteolytic damage.

Biochem/physiol Actions

Cell permeable: no

Kd = 5 x 10-14 M, 2.3 x 10-10 M, 1 x 10-7 M against trypsin, plasmin, and kallikrein, respectively

Primary Target
chymotrypsin

Product does not compete with ATP.

Reversible: yes

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One TIU (Trypsin Inhibitory Unit) will decrease the activity of two trypsin units by 50% where one trypsin unit will hydrolyze 1.0 µmole of Nα-benzoyl-L-Arginine-p-Nitroanilide (L-BAPNA) per minute at pH 7.8 at 25°C.

Physical form

Contains no animal-derived components.

Other Notes

Deutscher, M.P., 1990. Methods Enzymol.182, 83.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable