567370

Smac-N7 Peptide

A peptide that contains the N-terminal seven residues of Smac (Second Mitochondria-derived Activator of Caspases, also known as DIABLO) and promotes procaspase-3 activation at around 10 µM.

• Synonym(s): Smac-N7 Peptide, H-AVPIAQK-OH
• Empirical Formula (Hill Notation): C₃₃H₅₉N₉O₉
• Molecular Weight: 725.88
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• assay: ≥95% (HPLC)
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white
• solubility: water: 1 mg/mL; DMSO: 5 mg/mL
• shipped in: ambient
• storage temp.: −20°C

Description

General description

A peptide that contains the N-terminal seven residues of Smac (Second Mitochondria-derived Activator of Caspases, also known as DIABLO) and promotes procaspase-3 activation at around 10 µM. Reported to bind to the BIR2 and BIR3 (baculovirus IAP repeat) domains of XIAP (X-linked inhibitor of apoptosis proteins) with a K_d ~800 nM. Promotes the catalytic activity of caspase-9 in the presence of XIAP, suggesting that Smac competes with caspase-9 for binding to the same pocket on the surface of XIAP.

A peptide that contains the N-terminal seven residues of Smac (Second mitochondria-derived activator of caspases, also known as DIABLO) and promotes pro-caspase-3 activation at ~10 µM. Reported to bind to the BIR2 and BIR3 (baculovirus IAP repeat) domains of XIAP (X-linked inhibitor of apoptosis proteins) (K_d = ~800 nM). Promotes the catalytic activity of caspase-9 in the presence of XIAP, suggesting that Smac competes with caspase-9 for binding to the same pocket on the surface of XIAP.

Biochem/physiol Actions

Cell permeable: no

Kd ~800 nM in binding to the BIR2 and BIR3 (baculovirus IAP repeat) domains of XIAP (X-linked inhibitor of apoptosis proteins)

Primary Target
Promotes the catalytic activity of caspase-9 in the presence of XIAP

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

H-Ala-Val-Pro-Ile-Ala-Gln-Lys-OH

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Srinivasula, S.M., et al. 2001. Nature410, 112.
Chai, J., et al. 2000. Nature406, 855.
Liu, Z., et al. 2000. Nature408, 1004.
Srinivasula, S.M., et al. 2000. J. Biol. Chem.275, 36152.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable