Recognizes the ~87 kDa rSec6 protein in PC12 cells.
This Anti-rSec6 Mouse mAb (9H5) is validated for use in Immunoblotting, Immunofluorescence for the detection of rSec6.
Immunogen
Rat
recombinant, rat rSec6 fused to a His•Tag sequence
Application
Immunoblotting (4 µg/ml, chemiluminescence)
Immunofluorescence (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In PBS, 0.1 mM PMSF, 50% glycerol, pH 7.2.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control ESK-4 cells
Other Notes
Immunofluorescence of PC12 cells using this antibody shows colocalization of rSec6 with vesicles and sites of exocytosis. Variables associated with assay conditions will dictate the proper working dilution.
Kee, Y., et al. 1997. Proc. Natl. Acad. Sci. USA94, 14438. Hsu, S.-C., et al. 1996. Neuron17, 1209. TerBush, D.R., et al. 1995. J. Cell Biol.130, 299.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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