540221
Purine Nucleoside Phosphorylase, Human, Recombinant, E. coli
Synonym(s):
PNP
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.42
Recommended Products
recombinant
expressed in E. coli
Quality Level
form
liquid
specific activity
≥25 units/mg protein
manufacturer/tradename
Calbiochem®
shipped in
dry ice
storage temp.
−20°C
General description
Recombinant, human purine nucleotide phosphorylase expressed in E. coli without tags. Purine nucleoside phosphorylase catalyzes the phosphorlysis of N-ribosidic bonds of purine nucleosides and deoxynucleosides in a reversible reaction. Human PNP is a target for therapeutic T-cell immune response intervention. Useful in the detection of inorganic phosphate from biochemical reactions.
Recombinant, human purine nucleotide phosphorylase expressed in E. coli without tags. Purine nucleoside phosphorylase catalyzes the phosphorolysis of N-ribosidic bonds of purine nucleosides and deoxynucleosides. This reaction is reversible. The human enzyme is a target for therapeutic immune response intervention.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will convert 1 µmol of MESG and 1 µmol of Pi into 1 µmol of 2-amino-6-mercapto-7-methylpurine and 1 µmol of ribose phosphate in 1 min at 25°C, pH 7.6.
Physical form
In 50 mM Tris-HCl, pH 7.6.
Analysis Note
Single band by SDS-PAGE
Other Notes
Filgueira de Azevedo, W.Jr., et al. 2003. Biochem. Biophys. Res. Commun.309, 917.
Silva, R.G., et al. 2003. Protein Expr. Purif.27, 158.
Silva, R.G., et al. 2003. Protein Expr. Purif.27, 158.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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