539732-M

PTP, T-Cell, Human, Recombinant, E. coli

• Synonym(s): Protein Tyrosine Phosphatase, T-Cell
• UNSPSC Code: 12352202

Properties

• assay: ≥95% (SDS-PAGE)
• Quality Level: 100
• form: liquid
• specific activity: ≥10,000 units/mL; ≥15,000 U/mg
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• storage temp.: −70°C

Description

General description

Recombinant, human T-cell PTP, truncated at the C-terminus (an 11Kd deletion) and expressed in E. coli. Suitable for dephosphorylation of phosphotyrosine residues in proteins.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1 nmol of pNPP per min at 30°C, pH 7.0.

Physical form

In 100 mM NaCl, 50 mM HEPES, 5 mM DTT, 2 mM EDTA, 0.01% Brij^® 35 Detergent, 50% glycerol, pH 7.0.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Gordon, J.A. 1991. Methods Enzymol.201, 477.
Zander, N.F., et al. 1991. Biochemistry30, 6964.
Cool, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA86, 5257.

Legal Information

Brij is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable