5.38384

NCK/PAK Blocking Peptide

• Synonym(s): NCK/PAK Blocking Peptide, TAT-PAK peptide, NCK blocking peptide
• Empirical Formula (Hill Notation): C₁₂₆H₂₂₁N₅₁O₃₂S₂ · 11C₂HF₃O₂ · 7H₂O
• Molecular Weight: 4406.92
• UNSPSC Code: 12352200

Properties

• assay: ≥98% (HPLC)
• Quality Level: 100
• form: solid
• potency: 2 μM IC₅₀
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic); protect from light
• color: off-white
• solubility: water: 100 mg/mL
• storage temp.: −20°C

Description

General description

A cell-permeable synthetic peptide derived from the proline-rich region near the N-terminal of p21 activated kinase1 (PAK1) and fused with HIV TAT. Shown to selectively binds to the non-catalytic region of tyrosine kinase adaptor protein (NCK) and disrupts the cellular transport and localization of PAK. Shown to specifically inhibit endothelial cell migration and contractility (IC₅₀ = 2 µM) and block bFGF-induced angiogenesis in a chick chorioallantoic membrane (CAM) assay. Does not exhibit any deleterious effects on endothelial cell survival and growth.

Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
NCK

Reversible: yes

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

H2N-YGRKKRRQRRRGKPPAPPMRNTSTM-C02H

Physical form

Supplied as TFA salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.

Other Notes

Chen, J., et al. 2015, Sci. Signal.8, 365.

Kiosses, W., et al. 2002, Circ. Res.90, 697.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable