A cell-permeable tetrahydroquinolinedione[b]cyclobutene based compound that acts as a specific inhibitor of microRNA (miRNA)-221/222-myoD regulatory pathway. Inhibits the translation of myoD in C2C12 cells without changing the expression level of myoD mRNA, but reduces myoD protein expression levels. Shown to selectively inhibit miR-1 (IC50 = 2.5 µM), miR-133a and miR-206 (IC50 = 5 µM) in C2C12 cells. However, it does not affect the levels of non-myogenic miR-9, miR-25, miR-150, miR-25, and miR-214. Shown to inhibit the differentiation of C2C12 cells as indicated by the down-regulation of muscle creatine kinase, myosin heavy chain, and myoGenin levels in these cells.
Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.
Biochem/physiol Actions
Cell permeable: yes
Primary Target miR-221/22
Warning
Toxicity: Standard Handling (A)
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Tan, S., et al. 2014. Chem. & Biol.21, 1265.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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