5.32980

miR-34a Activator, Rubone

• Synonym(s): miR-34a Activator, Rubone, miroRNA-34a Activator, Rubone
• Empirical Formula (Hill Notation): C₂₀H₂₂O₇
• Molecular Weight: 374.38
• MDL number: MFCD00274772
• UNSPSC Code: 12352200

Properties

• assay: ≥98% (HPLC)
• Quality Level: 100
• form: powder
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: yellow
• solubility: DMSO: 10 mg/mL
• Storage temp.: −20°C
• SMILES string: O=C(C1=C(OC)C=C(OC)C=C1O)/C=C/C2=CC(OC)=C(OC)C=C2OC

Description

General description

A cell-permeable chalcone derivative that preferentially increases primary and mature miR-34a levels in Huh7 and HepG2 hepatocellular carcinoma cells (HCC) expressing wild-type or mutated p53, but does not affect miR-34a expression in non-tumorigenic Hep3B cells with deleted p53. Inhibits the luciferase activity in a HCC cell-based miR34a luciferase reporter system (IC₅₀ = 3.8 µM). Causes a significant reduction in the mRNA and protein levels of cyclin D1 and Bcl-2 in Huh7 and HepG2 cells (~ 10 µM), but not in Hep3B cells. Also shown to reduce the levels of miR-34a targets (Cdk6, FOXP, Notch 1 and SIRT) and p53 targets (p21 and PUMA) in Huh7 and HepG2 cells, but not in Hep3B cells. Inhibits the growth of multiple HCC by inducing apoptosis and reducing proliferation, but shows no toxicity on non-tumorigenic human hepatocytes. Suppresses the growth of Huh7 and HepG2 xenografts in nude mice model (~90% inhibition at 50 mg/kg).

Please note that the molecular weight for this compound is batch-specific due to variable water content.

A cell-permeable compound that preferentially increases primary and mature miR-34a levels in Huh7 and HepG2 cells expressing wild-type or mutated p53.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
miR-34a

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Xiao, Z., et al. 2014. Cancer Res.74, 6236.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable