524739

Phosphodiesterase 10A2, His•Tag^®, Human, Recombinant, S. frugiperda

• Synonym(s): PDE10A2
• Enzyme Commission number: 3.1.4.17 (BRENDA, IUBMB)
• UNSPSC Code: 12352202
• NACRES: NA.54

Properties

• recombinant: expressed in baculovirus infected Sf21 cells
• Quality Level: 100
• form: liquid
• specific activity: ≥0.25 U/μL
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −70°C

Description

General description

Full-length, recombinant, human, phosphodiesterdase 10A2 (accession no. 10A2) fused at the N-terminus to a His•Tag^7reg; sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. Supplied as a partially purified preparation of catalytically active enzyme.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme required to convert 1 µmol cAMP to AMP per min at 30°C, pH 7.5.

Physical form

In RIPA buffer (150 mM NaCl, 10 mM Tris-HCl, 1% NP-40), pH 8.3.

Other Notes

Kotera, J., et. al. 2004. J. Biol. Chem.279, 4366.
Fujishige K., et. al. 1999. Eur. J. Biochem.266, 1118.
Kotera, J., et. al. 1999. Biochem. Biophys. Res. Commun.261, 551.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable