521215
PDGF, AA Homodimer, Human, Recombinant, E. coli
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About This Item
UNSPSC Code:
12352202
Recommended Products
assay
≥97% (SDS-PAGE)
Quality Level
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
≤1 EU/μg Endotoxin (EU/μg PDGF-AA)
shipped in
ambient
storage temp.
−70°C
General description
Recombinant, human platelet derived growth factor AA (disulfide-linked dimer of two A-chain monomers) expressed in E. coli. Amino acid sequence is the long form of mature human PDGF-A chain and is identical to that deduced from the native human nucleotide sequence except for the addition of an N-terminal methionine group.
Biochem/physiol Actions
1-3 ng/ml
Warning
Toxicity: Standard Handling (A)
Physical form
Lyophilized from a sterile-filtered solution in 30% acetonitrile, 0.1% TFA.
Reconstitution
Following reconstitution, aliquot and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at 4°C.
Reconstitute to a concentration ≥10 µg/ml with sterile 4 mM HCl containing at least 0.1% HSA or BSA.
Other Notes
Butt, A.M., et al. 1997. J. Neurosci. Res. 48, 588.
Westermark, B., and Heldin, C.-H. 1991. Cancer Res.51, 5087.
Betsholtz, C., et al. 1986. Nature320, 695.
Raines, E.W., et al. 1985. Methods Enzymol.109, 749.
Westermark, B., and Heldin, C.-H. 1991. Cancer Res.51, 5087.
Betsholtz, C., et al. 1986. Nature320, 695.
Raines, E.W., et al. 1985. Methods Enzymol.109, 749.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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