5.06075

Novel Inducer of Death-1, NID-1

• Synonym(s): Novel Inducer of Death-1, NID-1, (E)-N-(2-ethylphenyl)-3-(5-nitrothiophen-2-yl)acrylamide
• Empirical Formula (Hill Notation): C₁₅H₁₄N₂O₃S
• CAS Number: 476316-58-2
• Molecular Weight: 302.35
• UNSPSC Code: 12352200

Properties

• assay: ≥98% (HPLC)
• Quality Level: 100
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: yellow
• solubility: DMSO: 50 mg/mL
• storage temp.: 2-8°C

Description

General description

A cell-permeable, nitrothiophenylpropenamide compound that induces active, energy-dependent, and rapid non-apoptotic cell death (EC₅₀ = 1.65 µM) in several transformed (DRD, RKO, H1299) and non-transformed (BJeH ) human cells, in a variety of cancer cell lines, and in mouse embryonic fibroblasts. The cell death appears to be non-necrotic, non-oxidative and is independent of DNA damage or cell proliferation. NID-1 induced cell death, although distinct from macroautophagy, does show PI 3-kinase suppression, ATG5-dependence, p62 degradation, and higher LC3 levels. Also, NID-1-induced death is independent of caspases, Bax and Bak and requires lysosomal activity and cytosolic vacuolization. Rescues mutant-huntingtin-induced cell death in a cell-based model of Huntington’s disease. Shown to increase cathepsin L levels, but does not affect cathepsins B, C, and H.

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Varma, H., et al. 2013. Exp. Cell Res.319, 1759.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable