487600-M

p-Nitrophenyl Phosphate, Disodium Salt, Tablets - CAS 4264-83-9 - Calbiochem

Each 175 mg tablet contains 5 mg of p-Nitrophenyl Phosphate, Disodium Salt plus an inert filler.

• Synonym(s): p-Nitrophenyl Phosphate, Disodium Salt, Tablets - CAS 4264-83-9 - Calbiochem, pNPP, 2Na
• Empirical Formula (Hill Notation): C₆H₄NO₆P · 2Na · 6H₂O
• CAS Number: 4264-83-9
• Molecular Weight: 371.14
• MDL number: MFCD00007319
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• form: tablet
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated
• color: light yellow
• storage temp.: 2-8°C
• InChI: 1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2
• InChI key: VIYFPAMJCJLZKD-UHFFFAOYSA-L

Description

General description

Each 175 mg tablet contains 5 mg of p-Nitrophenyl Phosphate, Disodium Salt (Cat. No. 4876) plus an inert filler. Dissolve one tablet in 5 ml of 10 mM diethanolamine, pH 9.5 containing 500 µM MgCl₂, to prepare a 1 mg/ml solution.

Excellent substrate for alkaline phosphatase-based ELISA assays. Produces a yellow soluble end product that can be read at 405 nm to 410 nm. Both kinetic and endpoint determinations can be performed. Each tablet contains 5 mg of p-Nitrophenyl Phosphate, Disodium Salt (cat. no. 4876) plus an inert filler.

Note: 1 each = 100 tablets.

Biochem/physiol Actions

Primary Target
Substrate for phosphatase

Warning

Toxicity: Standard Handling (A)

Preparation Note

Dissolve one tablet in 5 ml of of 10 mM diethanolamine, pH 9.5 containing 0.5 mM MgCl₂ to prepare a 1 mg/ml stock solution.

Reconstitution

Unstable in solution; reconstitute just prior to use. Following reconstitution, aliquot and refrigerate (4°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable