483400-M
7-Nitroindazole - CAS 2942-42-9 - Calbiochem
Synonym(s):
7-Ni
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Pricing and availability is not currently available.
assay
≥97% (TLC)
form
solid
color
yellow-brown
solubility
methanol: 5 mg/mL
DMSO: 50 mg/mL
General description
A cell-permeable, reversible and competitive inhibitor of nitric oxide synthase (NOS) with high selectivity for the brain enzyme (IC50 = 710 nM) vs. iNOS (IC50 = 20 μM). Also inhibits bovine endothelial nitric oxide synthase (IC50 = 800 nM). Binds to the heme group of NOS.
Biochem/physiol Actions
Target IC50:710 nM against the brain enzyme nitric oxide synthase (NOS)
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
From Catalog:
Desc. Field- added "cell-permeable"
From Catalog:
Desc. Field- added "cell-permeable"
Storage Class
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
P Klatt et al.
The Journal of biological chemistry, 269(19), 13861-13866 (1994-05-13)
Nitric oxide (NO) synthases contain FAD, FMN, heme, and (6R)-5,6,7,8-tetrahydro-L-biopterin as prosthetic groups. We have characterized the pteridine-binding site of purified brain NO synthase, using 3H-labeled (6R)-5,6,7,8-tetrahydro-L-biopterin as radioligand. Association of [3H]tetrahydrobiopterin followed second-order kinetics (kon = 1.3 x 10(6)
R C Babbedge et al.
British journal of pharmacology, 110(1), 225-228 (1993-09-01)
1. 7-Nitro indazole (7-NI) produces potent inhibition of rat cerebellar nitric oxide synthase (NOS) with an IC50 of 0.9 +/- 0.1 microM (n = 6). NOS activity is dependent on the presence of both exogenous CaCl2 and NADPH. The inhibitory
P K Moore et al.
British journal of pharmacology, 108(2), 296-297 (1993-02-01)
7-Nitro indazole (7-NI) inhibits mouse cerebellar nitric oxide synthase (NOS) in vitro with an IC50 of 0.47 microM. Following i.p. administration in mice, 7-NI (10-50 mg kg-1) produces dose-related anti-nociception as evidenced by an inhibition of late phase (15-30 min)
J B Schulz et al.
Journal of neurochemistry, 64(2), 936-939 (1995-02-01)
Several studies suggest that nitric oxide (NO.) contributes to cell death following activation of NMDA receptors in cultured cortical, hippocampal, and striatal neurons. In the present study we investigated whether 7-nitroindazole (7-NI), a specific neuronal nitric oxide synthase inhibitor, can
D J Wolff et al.
Archives of biochemistry and biophysics, 314(2), 360-366 (1994-11-01)
Citrulline formation by the Ca2+ CaM-dependent nitric oxide synthase of bovine endothelium is inhibited reversibly by 7-nitroindazole, 1-phenylimidazole, and imidazole. As measured at 0.67 microM (6R)-5,6,7,8-tetrahydrobiopterin (BH4), IC50 values of 0.8, 200, and 50 microM were determined for 7-nitroindazole, 1-phenylimidazole
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
