481001
Anti-Neutrophil Elastase Rabbit pAb
liquid, Calbiochem®
Synonym(s):
Neutrophil elastase antibody, Rabbit monoclonal antibody
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1 ML
$204.00
About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
Recommended Products
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
wet ice
Storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ELANE(1991)
General description
Ammonium sulfate precipitated rabbit polyclonal antibody. Recognizes human neutrophil elastase.
Recognizes the neutrophil elastase protein.
This Anti-Neutrophil Elastase Rabbit pAb is validated for use in ELISA, Immunoblotting, Immunoelectrophoresis, Immunoprecipitation for the detection of Neutrophil Elastase.
Immunogen
Human
human neutrophil elastase
Application
ELISA (≥ 1:1000)
Immunoblotting (see comments)
Immunoelectrophoresis (see comments)
Immunoprecipitation (see comments)
Immunoblotting (see comments)
Immunoelectrophoresis (see comments)
Immunoprecipitation (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In PBS, pH 7.5.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Single arc by immunoelectrophoresis against neutrophil granule extract and human neutrophil elastase. This antibody has also been reported to work for immunoblotting and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
12 - Non Combustible Liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Avinash Khandagale et al.
Journal of leukocyte biology, 104(6), 1199-1213 (2018-08-15)
Mutations in the gene JAGN1 were recently discovered in patients with severe congenital neutropenia (SCN). Neutrophils release neutrophil extracellular traps (NETs) consisting of decondensed chromatin decorated with various granular proteins such as neutrophil elastase and myeloperoxidase (MPO) to combat microbial
Ava Hosseinzadeh et al.
Frontiers in immunology, 3, 391-391 (2012-12-28)
To prevent the spread of pathogens neutrophils as the first line of defense are able to release Neutrophil Extracellular Traps (NETs), a recently discovered form of immune response. Reactive oxygen species (ROS) have been shown to be essential for many
Elaine F Kenny et al.
eLife, 6 (2017-06-03)
Neutrophils release neutrophil extracellular traps (NETs) which ensnare pathogens and have pathogenic functions in diverse diseases. We examined the NETosis pathways induced by five stimuli; PMA, the calcium ionophore A23187, nigericin, Candida albicans and Group B Streptococcus. We studied NET
Dae-goon Yoo et al.
Journal of immunology (Baltimore, Md. : 1950), 192(10), 4728-4738 (2014-04-18)
Cystic fibrosis (CF) airways are characterized by bacterial infections, excess mucus production, and robust neutrophil recruitment. The main CF airway pathogen is Pseudomonas aeruginosa. Neutrophils are not capable of clearing the infection. Neutrophil primary granule components, myeloperoxidase (MPO) and human
Volker Brinkmann et al.
Frontiers in immunology, 3, 413-413 (2013-01-15)
Neutrophil Extracellular Traps (NETs) consist of decondensed chromatin studded with granular and some cytoplasmic proteins. They are formed by activated neutrophil granulocytes, also called polymorphonuclear leukocytes (PMN) as the result of an active cell death program, named NETosis. NETosis can
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