475858-M

Met30 Antagonist, SMER3

The Met30 Antagonist, SMER3 controls the biological activity of Met30. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.

• Synonym(s): Met30 Antagonist, SMER3, Small-Molecule Enhancer of Rapamycin-3, 9H-Indeno[1,2-b][1,2,5]oxadiazolo[3,4-e]pyrazin-9-one, Indeno[1,2-b]furazano[3,4-E]pyrazin-9-one, SCF ^Met30 Inhibitor
• Empirical Formula (Hill Notation): C₁₁H₄N₄O₂
• Molecular Weight: 224.18
• UNSPSC Code: 12352200

Properties

• assay: ≥97% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: yellow
• solubility: DMSO: 10 mg/mL
• storage temp.: 2-8°C

Description

General description

A cell-permeable indenofurazinopyrazinone compound that is shown to directly target the F-box protein Met30 subunit of the Skp1-cullin-F-box (SCF) E3 ubiquitin ligase complex, disrupting Met30-Skp1 interaction and selectively inhibiting SCF^Met30, but not SCF^Cdc4, E3 ubiquitin ligase activity. SMER3 is reported to act in a synthetic lethal fashion with rapamycin (Cat. Nos. 553210, 553211, & 553212) in both human lung cancer A549 and in yeast cultures. SMER3 treatment (10 to 30 µM) results in cell cycle arrest and morphological phenotype resembling that seen with temperature-sensitive Met30, but not Skp1 or Cdc4, mutant under non-permissive temperature in yeast cultures.

Warning

Toxicity: Regulatory Review (Z)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

It is recommended to prepare a stock solution for 3 month use each time and store the unreconstituted material in solid form, protected from moisture and preferably under inert gas, for best long-term stability during storage.

Other Notes

Aghajan, M., et al. 2010. Nature Biotechnol.28, 738.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable