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411483

Sigma-Aldrich

Mouse Anti-Human IgG₃, Hinge Specific (HP6047) Biotin Conjugate

liquid, clone HP6047, Calbiochem®

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200 μG
$250.00

$250.00

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200 μG
$250.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.77

$250.00

Check Cart for Availability

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

secondary antibodies

clone

HP6047, monoclonal

form

liquid

does not contain

preservative

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG3

shipped in

ambient

storage temp.

−20°C

target post-translational modification

unmodified

Related Categories

General description

Purified mouse monoclonal antibody conjugated to biotin. Recognizes human IgG3 hinge region.
This Mouse Anti-Human IgG₃, Hinge Specific (HP6047) Biotin Conjugate is validated for use in ELISA for the detection of Human IgG₃, Hinge Specific.

Immunogen

Human

Application

ELISA (1:500-1:2000, see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS, 1% BSA, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Biotinylated with hydroxysuccinimide ester biotin. This biotin-labeled antibody is an extremely versatile reagent because of the high binding affinity for avidin and avidin-labeled compounds. Variables associated with assay conditions will dictate the proper working dilution.



Cross-reactivity by ELISA against human myeloma proteins:

Human IgG1: ≤0.1%

Human IgG2: ≤1%

Human IgG3: 100%

Human IgG4: ≤0.1%

Human IgA: ≤0.1%

Human IgE: ≤0.1%

Human IgM: ≤0.1%



Cross-reactivity by ELISA against chimeric antibodies:

Human IgG1: <0.001%

Human IgG2: <0.001%

Human IgG3: 100%

Human IgG4: <0.001%

Human IgA: <0.001%

Human IgE: <0.001%

Human IgM: <0.001%
Hamilton, R.G. 1990. Ann. Biol. Clin.48, 473.
Fasullo, F.J., et al. 1989. Clin. Chem.35, 364.
Hamilton, R.G. 1987. Clin. Chem.33, 1707.
Jefferis, R., et al. 1986. Immunol. Letters10, 223.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kelly E Seaton et al.
JCI insight, 2(24) (2017-12-22)
Accurate HIV-1 incidence estimation is critical to the success of HIV-1 prevention strategies. Current assays are limited by high false recent rates (FRRs) in certain populations and a short mean duration of recent infection (MDRI). Dynamic early HIV-1 antibody response
David R Martinez et al.
Cell, 178(1), 190-201 (2019-06-18)
The placental transfer of maternal IgG is critical for infant protection against infectious pathogens. However, factors that modulate the placental transfer of IgG remain largely undefined. HIV-infected women have impaired placental IgG transfer, presenting a unique "disruption model" to define

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