382184
Histone Deacetylase 8, His•Tag®, Human Recombinant, S. frugiperda
Histone Deacetylase 8, His•Tag, Human Recombinant, S. frugiperda, is a full length HDAC8 fused to His-Tag at the C-terminus. Useful in the study of HDAC8 regulation and for inhibitor screening.
Synonym(s):
HDAC8, Human, Recombinant
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50 μG
$748.00
$748.00
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50 μG
$748.00
About This Item
UNSPSC Code:
12352202
NACRES:
NA.54
$748.00
Check Cart for Availability
recombinant
expressed in insect cells (S. frugiperda)
Quality Level
assay
≥90% (SDS-PAGE)
form
liquid
specific activity
≥10 units/μg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−70°C
General description
Full length, recombinant, human histone deacetylase 8 (HDAC8) fused to at the C-terminus to a His•Tag sequence at and expressed in S. frugiperda insect cells using a baculovirus expression system. HDAC8 phosphorylated in vivo by PKA results in decreased activity, which leads to the hyperacetylation of histones H3 and H4. HDAC8 may play a role in one of the most frequent types of acute myeloid leukemia (AML). The active deacetylase is useful in the study of HDAC8 regulation and for inhibitor screening.
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the the amount of enzyme required to deacetylate 1 picomol fluorescent peptide substrate [Arg-His-Lys(Ac)-Lys(Ac)] per min at 30°C.
Physical form
In 138 mM NaCl, 25 mM Tris-HCl, 10% glycerol, 0.05% TWEEN®-20 detergent, pH 8.0.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Waltregny, D., et al. 2005. FASEB J.19, 966.
Buggy, J.J., et al. 2000. Biochem J. 350, 199
Buggy, J.J., et al. 2000. Biochem J. 350, 199
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
Certificates of Analysis (COA)
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