375230
HGF, Mouse, Recombinant
HGF, Mouse, Recombinant Recombinant, consists of disulfide-linked heterodimeric α (Gln33 to Arg495) and β (Val496 to Leu728) chains expressed in murine myeloma NS0 cell line.
Synonym(s):
Hepatopoietin A
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About This Item
UNSPSC Code:
12352203
Quality Level
assay
≥95% (as seen by a 60 kDa (α chain) and a 33 kDa (β chain) bands under reducing conditions., SDS-PAGE)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
<1.0 EU/μg protein Endotoxin
shipped in
ambient
storage temp.
−20°C
General description
Recombinant, mouse hepatocyte growth factor (HGF) consisting of disulfide-linked heterodimeric α (Gln33 to Arg495) and β (Val496 to Leu728) chains expressed in murine myeloma NS0 cell line.
Biochem/physiol Actions
Cell Proliferation:
ED₅₀ = 10-30 ng/ml as measured in a cell proliferation assay using IMCD-3 mouse epithelial cells.
Functional ELISA
KD<0.2 nM as measured by binding to C-Met (HGF Receptor).
ED₅₀ = 10-30 ng/ml as measured in a cell proliferation assay using IMCD-3 mouse epithelial cells.
Functional ELISA
KD<0.2 nM as measured by binding to C-Met (HGF Receptor).
Warning
Toxicity: Standard Handling (A)
Reconstitution
Reconstitute to 10 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.
Other Notes
Corso, S., et al. 2005. Trends Mol. Med.11, 284.
Karihaloo, A., et al. 2005. Nephron Exp. Nephrol.100, e40.
Urbanek, K., et al. 2005. Circ. Res.97, 663.
Hammond, D.E., et al. 2004. Curr. Top. Microbiol. Immunol.286, 21.
Rosario, M. and W. Birchmeier. 2004. Dev. Cell7, 3.
Gheradi, E., et al. 2003. Proc. Natl. Acad. Sci.100, 12039.
Chiu, S.J., et al. 2002. J. Biomed. Sci.9, 261.
Tanaka, Y., et al. 2002. Biochem. Biophys. Res. Commun.299, 472.
Maeshima, A., et al. 2000. Kid. Int.58, 1511.
Lesk, A.M. and W.D. Fordham. 1996. J. Mol. Biol.258, 501.
Saelman, E.U.M., et al. 1995. J. Cell Sci.108, 3531.
Mineo, R., et al. 1994. Endocrinology145, 4355.
Mizuno, K., et al. 1994. J. Biol. Chem.269, 1131.
Sasaki, M., et al. 1994. Biochem. Biophys. Res. Commun.199, 772.
Montesano, R., et al. 1991. Cell67, 901.
Karihaloo, A., et al. 2005. Nephron Exp. Nephrol.100, e40.
Urbanek, K., et al. 2005. Circ. Res.97, 663.
Hammond, D.E., et al. 2004. Curr. Top. Microbiol. Immunol.286, 21.
Rosario, M. and W. Birchmeier. 2004. Dev. Cell7, 3.
Gheradi, E., et al. 2003. Proc. Natl. Acad. Sci.100, 12039.
Chiu, S.J., et al. 2002. J. Biomed. Sci.9, 261.
Tanaka, Y., et al. 2002. Biochem. Biophys. Res. Commun.299, 472.
Maeshima, A., et al. 2000. Kid. Int.58, 1511.
Lesk, A.M. and W.D. Fordham. 1996. J. Mol. Biol.258, 501.
Saelman, E.U.M., et al. 1995. J. Cell Sci.108, 3531.
Mineo, R., et al. 1994. Endocrinology145, 4355.
Mizuno, K., et al. 1994. J. Biol. Chem.269, 1131.
Sasaki, M., et al. 1994. Biochem. Biophys. Res. Commun.199, 772.
Montesano, R., et al. 1991. Cell67, 901.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
Certificates of Analysis (COA)
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