371723
Anti-Giα-1 and Giα-2-Subunits, C-Terminal (345-354 and 346-355) Rabbit pAb
liquid, Calbiochem®
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About This Item
UNSPSC Code:
12352203
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
does not contain
preservative
species reactivity (predicted by homology)
mammals
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
wet ice
storage temp.
−70°C
target post-translational modification
unmodified
Gene Information
human ... GNAI1(2770)
General description
Anti-Giα-1 and Giα-2-Subunits, C-Terminal (345-354 and 346-355), rabbit polyclonal, recognizes Giα-1 and Giα-2 subunits. It is validated for use in Western blotting.
Protein A purified rabbit polyclonal antibody. Recognizes Giα-1 and Giα-2 subunit proteins.
Recognizes Giα-1 and Giα-2 subunits. Does not cross-react with G3α-3 or Goα subunits.
Immunogen
a synthetic peptide [(C)KNNLKDCGLF] corresponding to amino acids at the C-terminus of mammalian Giα-1 and Giα-2, conjugated to KLH
Application
Immunoblotting (1:1000)
Warning
Toxicity: Standard Handling (A)
Physical form
In 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Does not cross-react with Giα-3 and Goα. The specificity and cross-reactivity were confirmed with lysates from separate cultures of bacteria transfected with the genes for Giα-1, Giα-2, Giα-3, and Goα. Variables associated with assay conditions will dictate the proper working dilution.
Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol.195, 215.
Goldsmith, P.P., et al. 1987. J. Biol. Chem.262, 14683.
Goldsmith, P.P., et al. 1987. J. Biol. Chem.262, 14683.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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