345872

Glial Derived Neurotrophic Factor, Human, Recombinant, E. coli

• Synonym(s): rhGDNF
• UNSPSC Code: 12352202

Properties

• assay: ≥97% (SDS-PAGE)
• Quality Level: 100
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated
• solubility: aqueous buffer: soluble (most); water: soluble
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Recombinant, human glial derived neutotrophic factor expressed in E. coli. A heterogeneously glycosylated growth promoting factor that enhances neuronal survival of dopaminergic midbrain neurons. Activates receptor tyrosine kinase Ret and promotes kidney morphogenesis. Also an activator of MAP kinase. Its biological activity is destroyed by reduction of disulfide bonds.

Recombinant, human glial derived neutotrophic factor expressed in E. coli. Growth promoting factor that enhances neuronal survival of dopaminergic midbrain neurons. A heterogeneously glycosylated basic protein. Its biological activity is destroyed by the reduction of disulfide bonds.

Biochem/physiol Actions

fully biologically active when compared to native GDNF by dose-dependent ³H-dopamine uptake assay

Warning

Toxicity: Standard Handling (A)

Preparation Note

The recommended stock solution is 1 µg/100µl. For more dilute samples, add a stabilizing protein such as 0.1% BSA.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Vega, Q.C., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 10657.
Worby, C.A., et al. 1996. J. Biol. Chem. 271, 23619.
Lin, L.F., et al. 1994. J. Neurochem. 63, 758.
Lev-Fen, H., et al. 1993. Science260, 1130.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany