324762

Endopeptidase, Neutral, Porcine Kidney

• Synonym(s): CD10, Common Acute Lymphocytic Antigen, Endopeptidase-24.11, Enkephalinase, Microvillus Proteinase, Neprilysin
• EC Number: 3.4.24.11 (BRENDA, IUBMB)
• UNSPSC Code: 12352202

Properties

• assay: ≥95% (HPLC)
• form: lyophilized
• specific activity: ≥850 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• solubility: physiological buffer, pH 7.4: 1 mg/mL (e.g., Tris-buffered saline)
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Native, neutral endopeptidase from porcine kidney. A cell surface zinc-containing proteinase that is abundant in the brush border membranes of the kidney proximal tubules. Also present in the brain, endothelial cells, liver, and the lung tissue. Peptidase activity is specifically directed towards the cleavage on the amino side of hydrophobic amino acid residues. Contains an arginine in the active site that can interact with the C-terminal carboxylate of substrates.

Native, neutral endopeptidase from porcine kidney. A cell surface, zinc containing metallopeptidase that is abundant in the brush border membrane of the kidney proximal tubules. Also present in brain, endothelial cells, liver, and lung. Peptidase activity is specifically directed toward the cleavage on the amino side of hydrophobic residues. Contains an arginine in the active site that can interact with the C-terminal carboxylate of substrates.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit of enzyme will liberate 1.0 µmol pNA per min from Glutaryl-(Ala)₂-Phe-pNA at 25°C, pH 7.4.

Physical form

Lyophilized from 150 mM NaCl, 25 mM Tris-HCl, 5 mM CaCl₂, TRITON^™ X-100 Detergent, pH 7.4.

Reconstitution

Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 3 days at 4°C.

Other Notes

Barnes, K., et al. 1995. J. Neurochem. 64, 1826.
Gafford, J.T., et al. 1983. Biochemistry 22, 3265.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable