324626

EGTA, Molecular Biology Grade

• Synonym(s): EGTA, Molecular Biology Grade, Ethyleneglycol- bis(β-aminoethyl)-N,N,Nʹ,Nʹ-tetraacetic Acid
• Empirical Formula (Hill Notation): C₁₄H₂₄N₂O₁₀
• CAS Number: 67-42-5
• Molecular Weight: 380.35
• MDL number: MFCD00004291
• UNSPSC Code: 12352106
• NACRES: NA.25

Properties

• grade: Molecular Biology
• Quality Level: 100
• assay: ≥98% dry basis (complexometric)
• form: solid (fine)
• reaction suitability: reagent type: chelator
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white
• solubility: 1 M NaOH: soluble
• cation traces: heavy metals (as Pb): ≤0.001%
• foreign activity: DNases, proteases, RNases, none detected
• shipped in: ambient
• storage temp.: 10-30°C
• InChI: 1S/C14H24N2O10/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24)
• InChI key: DEFVIWRASFVYLL-UHFFFAOYSA-N

Description

General description

Chelating agent selective for Ca²⁺. Binds one cation per molecule. Induces apoptosis in pheochromocytoma (PC12) cells.

Application

• Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor.: This study presents a genetically encoded biosensor for real-time monitoring of lysosomal pH changes, potentially enhanced by buffering agents like EGTA. The findings may offer insights into cellular mechanisms influenced by calcium chelation (Ponsford et al., 2021).


• Comparative Proteomic Analysis of Pleurotus ostreatus Reveals Great Metabolic Differences in the Cap and Stipe Development and the Potential Role of Ca(2+).: The research highlights the role of calcium in fungal development, with EGTA potentially influencing these biological processes through calcium chelation (Zhu et al., 2019).

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 1 year at 4°C.

Other Notes

Lindenboim, L., et al. 1995. J. Neurochem.64, 1054.
Bers, D.M. 1982. Am. J. Physiol.242, C404.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2