227041
Cholera Toxin, B Subunit, Type Inaba 569B, Peroxidase Conjugate
peroxidase conjugate, Suitable for the demonstration of dendritic branching in retrogradely labeled neurons.
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About This Item
UNSPSC Code:
12352202
Recommended Products
conjugate
peroxidase conjugate
Quality Level
form
solid
specific activity
≥125 purpurogallin units/mg protein (HRP)
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
shipped in
ambient
storage temp.
2-8°C
General description
Sold on the basis of HRP content.
Suitable for the demonstration of dendritic branching in retrogradely labeled neurons. Has been shown to be up to 50 times more sensitive than free peroxidase as both an orthogradely and retrogradely transported marker in the rat visual system.
Biochem/physiol Actions
Cell permeable: no
Primary Target
Demonstration of dendritic branching in retrogradely labeled neurons
Demonstration of dendritic branching in retrogradely labeled neurons
Product does not compete with ATP.
Reversible: no
Packaging
yes
Warning
Toxicity: Harmful (C)
Physical form
Lyophilized from 10 mM sodium phosphate buffer, pH 7.5.
Preparation Note
Prepared by a modification of the two-step conjugation method of Avrameas, S., and Ternynck, T. 1971. Chromatographically purified and lyophilized.
Reconstitution
Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 3 months at 4°C.
Reconstitute in ≤100 µl of sterile distilled H₂O.
Other Notes
Raappana, P., and Arvidsson, J. 1993. J. Comp. Neurol. 328, 103.
Trojanowski, J.Q. 1983. J. Neurosci. Methods9, 185.
Trojanowski, J.Q., et al. 1982. Brain Res.231, 33.
Wan, X.S.T., et al. 1982. Exp. Neurol.78, 167.
Avrameas, S., and Ternynck, T. 1971. Immunochemistry8, 1175.
Trojanowski, J.Q. 1983. J. Neurosci. Methods9, 185.
Trojanowski, J.Q., et al. 1982. Brain Res.231, 33.
Wan, X.S.T., et al. 1982. Exp. Neurol.78, 167.
Avrameas, S., and Ternynck, T. 1971. Immunochemistry8, 1175.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 1 Dermal - Acute Tox. 1 Oral - Resp. Sens. 1
Storage Class
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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