219404

Cathepsin H, Human Liver

Cathepsin H, Human Liver, CAS 60748-73-4, is native cathepsin H from human liver that functions both as an aminopeptidase and as an endopeptidase.

• Synonym(s): Cathepsin H, Human Liver, CTSH, cat H, procathepsin h
• CAS Number: 60748-73-4
• Enzyme Commission number: 3.4.22.16 (BRENDA, IUBMB)
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• Quality Level: 100
• form: liquid
• specific activity: ≥200 mU/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• technique(s): activity assay: suitable
• color: colorless
• pH: 4.5
• solubility: soluble; water: soluble
• suitability: suitable for Western blot
• NCBI accession no.: NM_004390
• UniProt accession no.: P09668
• shipped in: wet ice
• storage temp.: −70°C
• Gene Information: human ... CTSH(1512)

Description

General description

Research area: Cell signaling

Native Cathepsin H, from the human liver, is the only cathepsin with mono-aminopeptidase activity.

Application

Cathepsin H has been used to determine the potential of aziridinyl peptides to act as inhibitors of this protein and potentially explore their therapeutic applications.

Biochem/physiol Actions

Cathepsin H, derived from human liver, serves dual functions as both an aminopeptidase and an endopeptidase. It plays a significant role in the catabolism of proteins within lysosomes. Cathepsin H is classified as an endoaminopeptidase because it can hydrolyze proteins, N-terminal substituted proteins, amino acid derivatives, and peptides with different chain lengths and N-terminal free amino acid derivatives. This protein is also observed to exhibit amidase and esterase activity.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol H-Arg-βNA per min at 40°C, pH 6.8.

Physical form

In 100 mM NaCl, 20 mM sodium acetate buffer, 1 mM EDTA, pH 4.5.

Preparation Note

Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Note: 1 mU = 1 milliunit.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2