17-10451
CpGenome Direct Prep Bisulfite Modification Kit (50 Reactions)
The CpGenome Direct Prep Bisulfite Modification Kit allows bisulfite conversion directly from a variety of starting materials, including cultured cells, blood, fresh tissue & fixed tissue samples.
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84
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General description
Methylation of cytosines located 5′ to guanosine is known to have a profound effect on the expression of many eukaryotic genes. In normal cells methylation occurs predominantly in CG-poor regions, while CG-rich areas, called CpG-islands remain unmethylated. The exceptions are the extensive methylation of CpG islands associated with transcriptional inactivation of regulatory regions of imprinted genes and genes on the inactive X-chromosome of females. Aberrant methylation of normally unmethylated CpG islands has been documented as a relatively frequent event in immortalized and transformed cells and has been associated with transcriptional inactivation of defined tumor suppressor genes in human cancers. Hundreds of CpG islands are now known to exhibit the characteristic of hypermethylation in tumors resulting in identification of candidate genes that can be interrogated to determine extent of tumor specific transformation.
Unlike other bisulfite modifications approaches that start with isolated genomic DNA, the CpGenome Direct Prep Bisulfite Modification Kit allows simple and reliable bisulfite conversion of DNA directly from a variety of starting materials, including cultured cells, blood, fresh tissue and fixed tissue samples.
Key Features
Key Features
- Bisulfite conversion directly from cells, tissues, blood and FFPE samples without DNA purification
- Sensitive bisulfite-conversion from as few as 10 cells or as low as 50 pg of input DNA
- Fast and simple, streamlined protocol for one-step bisulfite conversion
- In-column desulfonation allows recovery of DNA without additional precipitation steps for more consistent results
- Suitable for downstream analysis by methlylation specific PCR, restriction digestion, sequencing, microarray hybridization, etc.
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Components
2X Extraction Buffer
Modification Reagent
Conversion Buffer
Resuspension Buffer
Equilibration Buffer
Binding Buffer
Wash Buffer I
Wash Buffer II
Elution Buffer
Proteinase K
Proteinase K Storage Buffer
Desalting Columns
Collection Tubes
Modification Reagent
Conversion Buffer
Resuspension Buffer
Equilibration Buffer
Binding Buffer
Wash Buffer I
Wash Buffer II
Elution Buffer
Proteinase K
Proteinase K Storage Buffer
Desalting Columns
Collection Tubes
Linkage
Replaces: Replaces S7820 (CpGenome Universal DNA Modification Kit).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
8A - Combustible corrosive hazardous materials
Certificates of Analysis (COA)
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Li-Min Gao et al.
The American journal of surgical pathology, 40(6), 836-846 (2016-03-15)
Aggressive natural killer cell leukemia (ANKL) is a rare disease with an extremely aggressive clinical course. The etiology of ANKL is unclear with few genetic/epigenetic aberrations described to date. Moreover, misdiagnosis of ANKL is a frequent problem. Clinicopathologic characteristics of
B Leclercq et al.
Environmental pollution (Barking, Essex : 1987), 218, 1074-1088 (2016-09-07)
While the knowledge of the underlying mechanisms by which air pollution-derived particulate matter (PM) exerts its harmful health effects is still incomplete, detailed in vitro studies are highly needed. With the aim of getting closer to the human in vivo conditions and
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Wang, L; Ge, J; Ma, T; Zheng, Y; Lv, S; Li, Y; Liu, S
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Genetic and epigenetic alterations in normal and sensitive COPD-diseased human bronchial epithelial cells repeatedly exposed to air pollution-derived PM2.5.
Leclercq, B; Platel, A; Antherieu, S; Alleman, LY; Hardy, EM; Perdrix, E; Grova et al.
Environmental Pollution (Barking, Essex : 1987) null
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