16-454
Anti-Phosphotyrosine Antibody, clone PY20, HRP Conjugate
clone PY20, Upstate®, from mouse
Synonym(s):
PY20, anti-phosphotyrosine antibody
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
conjugate
peroxidase conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
PY20, monoclonal
species reactivity (predicted by homology)
all
manufacturer/tradename
Upstate®
technique(s)
ELISA: suitable
western blot: suitable
isotype
IgG2b
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PID1(55022)
General description
Protein tyrosine phosphorylation was first described in 1979. Large numbers of tyrosine kinases have been described since, and many of the signaling pathways initiated by tyrosine phosphorylation have been delineated. Availability of a top-quality anti-phosphotyrosine antibody has allowed the study of tyrosine kinase activity by simple blotting procedures, and without routine need of large quantities of radioactivity. The 4G10 monoclonal anti-phosphotyrosine antibody (Cat. # 05-321) has been widely recognized as a highly specific and sensitive. This antibody is suitable for all applications involving protein tyrosine phosphorylation. The slightly less sensitive, but still highly regarded clone PY20 can be used as an alternative to the 4G10 product line.
Specificity
Recognizes proteins containing phosphotyrosine residues, regardless of origin.
Immunogen
Epitope: phosphotyrosine
Phosphotyrosine conjugated to carrier protein
Application
Anti-Phosphotyrosine Antibody, clone PY20, HRP Conjugate is a Mouse Monoclonal Antibody for the detection of Phosphotyrosine & has been validated in WB, ELISA.
Research Category
Signaling
Signaling
Research Sub Category
General Post-translation Modification
General Post-translation Modification
Quality
Routinely evaluated by western blot on RIPA lysates from insulin-treated human HEK293 cells.
Target description
Varies
Physical form
100 μL of protein A purified mouse IgG2b in PBS containing 0.05% Kathon® and HRP stabilizing compounds. Sufficient for a minimum of 10 immunoblots, if used at the optimum concentration of 1:2000- 1:4000 in 10 mL blocking buffer. Liquid.
Protein A purified
Storage and Stability
6 months at 2-8°C from date of shipment.
NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Legal Information
Kathon is a registered trademark of Rohm and Haas Co.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Warning
hcodes
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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S F Retta et al.
Molecular biology of the cell, 9(4), 715-731 (1998-05-16)
The beta1-integrin cytoplasmic domain consists of a membrane proximal subdomain common to the four known isoforms ("common" region) and a distal subdomain specific for each isoform ("variable" region). To investigate in detail the role of these subdomains in integrin-dependent cellular
Becky K Brisson et al.
PloS one, 7(9), e45588-e45588 (2012-10-03)
Insulin-like growth factor-I (IGF-I) is an essential growth factor that regulates the processes necessary for cell proliferation, differentiation, and survival. The Igf1 gene encodes mature IGF-I and a carboxy-terminal extension called the E-peptide. In rodents, alternative splicing and post-translational processing
Julia Durzyńska et al.
Endocrinology, 154(3), 1215-1224 (2013-02-15)
IGF-I is a key regulator of muscle development and growth. The pre-pro-peptide produced by the Igf1gene undergoes several posttranslational processing steps to result in a secreted mature protein, which is thought to be the obligate ligand for the IGF-I receptor
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