1.50433

LiChrosorb^® RP-18 (5 µm) HPLC Columns

L x I.D. 125 mm x 4 mm, Hibar^® RT HPLC Column

• Synonym(s): TM=["LiChrosorb"] C18 HPLC column
• UNSPSC Code: 41115709
• NACRES: SB.52

Properties

• product name: LiChrosorb^® RP-18 (5 µm) Hibar^® RT 125-4, suitable for HPLC
• material: stainless steel column
• Quality Level: 100
• agency: suitable for USP L1
• product line: LiChrosorb^®
• feature: endcapped: no
• parameter: 400 bar pressure; 60 °C max. temp.
• technique(s): HPLC: suitable; mass spectrometry (MS): suitable (Low)
• column L × I.D.: 12.5 cm × 4 mm
• matrix: fully porous particle
• matrix active group: C18 bonding phase
• particle size: 5 μm
• pore size: 100 Å pore size
• operating pH range: 2-7.5
• separation technique: reversed phase; supercritical fluid (SFC)
• storage temp.: no temp limit

Description

General description

HPLC column

LiChrosorb^® RP-18 is a reliable irregular shaped silica gel carriers with reversed phase properties. It is well suited for the separation of neutral, acidic and weak basic compounds.This sorbent is packed into a Hibar 125-4 HPLC column. For mounting of a LiChroCART 4-4 guard column, the guard column holder 1.51487 has to be used.

Application

• Simultaneous determination of ampicillin and metampicillin in biological fluids using high-performance liquid chromatography with column switching.: This research introduced an HPLC method with column switching to simultaneously determine ampicillin and metampicillin in biological fluids. The use of LiChrosorb RP-8 (5 micro m) in this method enhanced the separation efficiency, crucial for therapeutic drug monitoring (Lee et al., 1995) (Lee et al., 1995).

Analysis Note

Pressure: ≤ 60 bar
Theoretical Plates (N/m) (Toluene): ≥ 55000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): 1.0 - 2.0

Legal Information

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany

LiChrosorb is a registered trademark of Merck KGaA, Darmstadt, Germany