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14-516-M

Sigma-Aldrich

Cdk5/p25 Protein, active, 10 µg

Active complex of N-terminal His6-tagged, recombinant, full-length human Cdk5 & N-terminal GST-tagged, recombinant, full-length human p25, for use in Kinase Assays.

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.32

biological source

human

Quality Level

recombinant

expressed in baculovirus infected Sf21 cells

mol wt

Mw 34.4 kDa (cdk5)
Mw 49.4 kDa (p25)

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

NCBI accession no.

UniProt accession no.

General description

Complex of N-terminal His6-tagged, recombinant, full-length human cdk5 and Nterminal GST-tagged, recombinant, full-length human p25
Product Source: Co-expressed by baculovirus in Sf21 insect cells

Application

Research Category
Metabolism
Research Sub Category
Obesity

Metabolic Disorders

Biochem/physiol Actions

Protein Target: CDK5/p25
Target Sub-Family: CMGC

Packaging

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-516M when ordering the 250 µg size.

Quality

routinely evaluated by phosphorylation of Histone H1

Physical form

Ni2+/NTA-agarose

Storage and Stability

1 year at -70°C

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Qiaoyun Chu et al.
Cancer medicine, 10(11), 3689-3699 (2021-05-08)
The marked overexpression of cyclin-dependent kinase 5 (CDK5) or Notch1 receptor, which plays critical roles in pancreatic ductal adenocarcinoma (PDAC) development, has been detected in numerous PDAC cell lines and tissues. Although, a previous study has demonstrated that CDK5 inhibition

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