14-415
Ral Assay Reagent (Ral BP1, agarose)
Synonym(s):
Agarose Ral Assay, Ral BP1 Assay Reagent, Ral Detection Reagent
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About This Item
UNSPSC Code:
12352204
eCl@ss:
32160405
NACRES:
NA.41
Recommended Products
form
liquid
Quality Level
manufacturer/tradename
Upstate®
technique(s)
affinity binding assay: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
General description
GST fusion-protein, corresponding to residues 397-519 of rat Ral binding protein (RalBP1) bound to agarose. Also referred to as RLIP and RLP; specifically binds to and precipitates RalA-GTP and RalB-GTP from cell lysates.
Product Source: Expressed in E. coli.
Quality
Routinely evaluated by Affinity Precipitation/Immunoblot Analysis
Physical form
300 μg of protein in 600 μL of a 50% agarose slurry in 50 mM Tris, pH 7.5, 200 mM NaCl, 1% Triton X-100, 20 μM PMSF, 1 mM aprotinin prior to the addition of glycerol to 50%. Liquid suspension.
Storage and Stability
1 year at -20°C
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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T Goi et al.
Molecular and cellular biology, 19(3), 1731-1741 (1999-02-18)
Ras proteins can activate at least three classes of downstream target proteins: Raf kinases, phosphatidylinositol-3 phosphate (PI3) kinase, and Ral-specific guanine nucleotide exchange factors (Ral-GEFs). In NIH 3T3 cells, activated Ral-GEFs contribute to Ras-induced cell proliferation and oncogenic transformation by
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