14-347-M

CHK2 Protein, active, 10 µg

Active, recombinant human CHK2, residues 5-543, containing N-terminal GST & C-terminal His-6 tags, for use in Kinase Assays.

• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in E. coli
• product line: Upstate^®
• specific activity: (For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)
• mol wt: M_w 89.6 kDa
• purified by: (glutathione-agarose and Ni²⁺/NTA-agarose)
• species reactivity: human
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable (kinase)
• accession no.: (FUNCTION: SwissProt: O96017 # Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on ′Ser-216′, preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at ′Thr-18′ and ′Ser-20′. ); NM_001005735.1
• NCBI accession no.: NM_001005735.1; NM_007194.3; NM_145862.2
• UniProt accession no.: O96017
• Gene Information: human ... CHEK2(11200)

Description

General description

Recombinant human CHK2, residues 5-543, containing N-terminal GST and Cterminal His6 tags

Biochem/physiol Actions

Protein Target: CHK2

Target Sub-Family: CAMK

Packaging

Also available in 10 μg size and in bulk

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-347M when ordering the 250 µg size.

Quality

routinely evaluated by phosphorylation of the CHKtide substrate peptide

Target description

CHK2

Physical form

glutathione-agarose and Ni²⁺/NTA-agarose

Storage and Stability

1 year at -70°C

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_c: Not applicable