14-1128
Di-Ubiquitin (Linear) Recombinant Protein | 14-1128
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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mol wt
observed mol wt ~17 kDa
observed mol wt ~34 kDa
technique(s)
western blot: suitable
shipped in
dry ice
General description
The function, processing and ultimate fate of polyubiquitinylated proteins is implied to be determined by the linkage between adjacent ubiquitin molecules in the polyubiquitin chain. The post-translational modification of proteins with polyubiquitin chains occurs in a wide range of signalling pathways and is tightly regulated in order to ensure cellular homeostasis. Several ubiquitin polygenes are encoded in eukaryotic cells and undergo post-translational processing to generate the cellular source of free ubiquitin monomers. However, linear polyubiquitin chains have been shown to be assembled in vitro by an E3 ligase complex, known as the linear ubiquitin chain assembly complex (LUBAC), and ubiquitin binding domains (UBDs) with a preference for linear polyubiquitin have been identified in a number of proteins. Recent studies suggest that linear polyubiquitin modification of proteins may play an important role in cellular processes in addition to that of lysine-linked polyubiquitin protein conjugation. Aditionally, studies suggest a possible role for linear polyubiquitin modification in NF-κB pathway activation, involving linear polyubiquitinylation of NEMO.
application
Liquid Chromatography - Mass Spectophotometry (LC-MS) Analysis: This product was subjected to LC-MS.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
Quality
Evaluated by Western Blotting using a panel of polyubiquitin proteins.
Western Blotting Analysis: 0.14 µg/µL of Cat. No. MABS199, Anti-linear Ubiquitin, clone 1E3 detected Di-Ubiquitin (Linear) Recombinant Protein in a panel of polyubiquitin proteins.
Western Blotting Analysis: 0.14 µg/µL of Cat. No. MABS199, Anti-linear Ubiquitin, clone 1E3 detected Di-Ubiquitin (Linear) Recombinant Protein in a panel of polyubiquitin proteins.
Physical form
Format: Purified
Liquid. 50 µg of Di-Ubiquitin reconstituted in 2.5 µL DMSO and 56 µL of 0.02 M TRIS, 0.25 M NaCl, pH 7.5 to a final concentration of 50 µM. General used concentrations vary from 10 – 50 μM. Buffer exchange using 3 kDa spin filters (or dialysis membrane) can be used to remove the DMSO if desired.
Storage and Stability
Store reconstituted solution at −80°C. Please avoid multiple freeze/thaw cycles.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
188.6 °F - (refers to pure substance)
flash_point_c
87 °C - (refers to pure substance)
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