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usage
sufficient for 20 sample(s) (60-180 μl each)
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
input
sample type plasma
sample type serum
sample type cerebrospinal fluid (CSF)
shipped in
wet ice
storage temp.
2-8°C
General description
High capacity, reusable columns specifically designed for the rapid and efficient removal of >80% of albumin from body fluid samples, either on an LC-instrument or manually (attached to a syringe). Removal of these high abundance proteins allows enhanced detection of low abundance proteins. Each column can be re-used up to 10 times.
Components
Two Albumin Removal Columns, 10X Albumin/IgG Binding Buffer, Albumin Elution Buffer, and a user protocol.
Warning
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Principle
The ProteoExtract Albumin Removal Kit (Maxi) provides a convenient, reproducible and highly specific method for removing serum albumin from 90 to 180 µl of plasma or serum. The kit provides columns containing a novel albumin-specific affinity resin. These columns allow for the removal of more than 80 % of serum albumin from human body fluid samples when used according to the instructions provided in this manual. The column hardware is designed to allow for both manual and LC-instrument controlled usage. When used manually, the albumin removal columns are operated as syringe-tip filters, when used with LC-instrument control, the columns can be connected to any standard HPLC/FPLC instrument via a 10/32" port using the provided adapters. For guidelines of ProteoExtract column connections and operation, please refer to section 3 of this manual.
Storage and Stability
Upon arrival store entire kit contents at 2-8°C. Avoid freezing of the Albumin Removal columns! Freezing thawing will destroy the affinity resin and the columns no longer function.
Other Notes
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PROTEOEXTRACT is a registered trademark of Merck KGaA, Darmstadt, Germany
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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