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Supelco

Chromolith® RP-18 endcapped (monolithic) HPLC Columns

L × I.D. 100 mm x 4.6 mm HPLC Column

Synonym(s):

Chromolith® Performance RP-18 endcapped 100-4.6 HPLC column

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1 UNIT
$1,160.00

$1,160.00

Available to ship onApril 30, 2025FromALLENTOWN

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1 UNIT
$1,160.00

About This Item

UNSPSC Code:
41115709
NACRES:
SB.52

$1,160.00

Available to ship onApril 30, 2025FromALLENTOWN

Product Name

Chromolith® RP-18 endcapped, L × I.D. 100 mm x 4.6 mm HPLC column

material

PEEK column

Quality Level

100

agency

suitable for USP L1

product line

Chromolith®

feature

endcapped

packaging

1 ea of

extent of labeling

18% Carbon loading

parameter

200 bar pressure
45 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)

column L × I.D.

10 cm × 4.6 mm

matrix

monolithic silica

matrix active group

C18 bonding phase

pore size

13 nm mesoporosity
2 μm macroporosity

operating pH range

2-7.5

separation technique

reversed phase

storage temp.

no temp limit

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General description

Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith® Performance RP-18 endcapped HPLC columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å) and the surface modification is octadecylsilane with full end-capping. Chromolith® Performance RP-18 endcapped columns fall under the USP L1 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column.

Chromolith® Performance RP-18 endcapped columns are perfectly suitable for LC-MS applications.
The column dimension of 100 mm length and 4.6 mm I.D. is the best choice for separations of complex samples on any HPLC System.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
  • Rapid separations at very low column back-pressure
  • High matrix tolerance, allowing time and cost savings with simplified workflows
  • Cost savings due to extended column lifetimes
  • Connection of columns, flow gradients and the use of mobile phases with higher viscosity


The recommended Guard-columns are:
Chromolith® RP-18 endcapped 5-4.6 guard cartridges (3 pieces) [1.51452] with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032] Or Chromolith® RP-18 endcapped 10-4.6 guard cartridges (3 pieces) [1.51451] with the Chromolith® Guard Cartridge Holder 10 mm x 4.6 mm [1.52033]

The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]

Need more efficiency?
Chromolith® HighResolution RP-18 endcapped 100-4.6 HPLC columns [1.52022] provide a column efficiency exceeding 140,000 plates/meter.

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Application


  • Salt pollution reduces turbidity, dissolved organic matter, and cyanobacteria in experimental vernal pool communities: Discusses environmental applications of calcium chloride, focusing on its use in managing ecosystems affected by salt pollution, demonstrating its role in environmental remediation and management. (Vigil and Schuler, 2024).



Analysis Note

Appearance: conforms
Carbon content: 17,0 - 18,6 %
Pressure: ≤ 30 bar
stability testing: conforms
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 80000
Capacity factor (Anthracene): 3,9 - 4,6
Tailing (USP): 0,90 - 1,60 %
Selectivity
separation factor (anthracene/progesterone): 1,95 - 2,35

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10-13 - German Storage Class 10 to 13

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Teriparatide Peptide Mapping Analysis by LC-UV-MS

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Monolithic Silica with Bimodal Pore Structure

Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.

Questions

1–3 of 3 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. What is the particle size of Chromolith 18 endcap column

    1 answer

    1. This product is not comprised of distinct particles; rather it is a a monolithic silica column. This silica monolith contains both mesopores (~13 nm) and macropores (~2 um) Please see the following for more information:
      https://www.sigmaaldrich.com/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/chromolith-hplc-and-uhplc-columns

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