1.00445

GranuCult^® plus Universal beer agar

for Brettanomyces/Dekkera spp., suitable for microbiology

• Synonym(s): UBA Medium
• UNSPSC Code: 41106213
• NACRES: NA.74

Properties

• sterility: non-sterile
• Quality Level: 200
• form: granular
• packaging: pkg of 500 g
• manufacturer/tradename: GranuCult^® plus
• storage condition: dry media (Keep tightly closed)
• technique(s): microbiological culture: suitable
• color: light brown
• pH: 6.1-6.5 (25 °C, 55 g/L in H₂O, after autoclaving)
• solubility: 73 g/L
• application(s): food and beverages; microbiology
• storage temp.: 15-25°C
• suitability: Acetobacter spp.; Brettanomyces/Dekkera spp.; Lactobacillus spp.; Pediococcus spp.

Description

General description

GranuCult^® plus Universal beer agar is a basal, non-selective agar rich in nutrients that support the growth and recovery of microorganisms. The incorporation of beer in the medium adds hop constituents and alcohol, which eliminate many airborne contaminants not originating from pitching yeasts, wort, or beer, thus minimizing false positive results. Also, it stimulates the growth of beer spoilage organisms, such as Lactobacilli, Pediococci, Acetobacter, Zymomonas spp., and wild yeast strains, which may be found infecting the pitching yeasts, the cooled wort or during fermentation or storage of the finished beer.

Cycloheximide (1 mg/L) may be added to detect bacterial contaminants in pitching yeasts.

Application

GranuCult^® plus Universal beer agar is suitable for the detection of beer spoilage microorganisms.

Features and Benefits

• GranuCult^® offers superior granulated culture media
• Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
• Excellent wettability, solubility, and free-flowing properties
• Convenient, with minimal component separation and clumping, even under warm or humid conditions
• High batch-to-batch reproducibility
• Prolonged shelf life of up to five years
• High number of test strains exceeding all regulatory demands
• Granulation technology allows many supplements to be included, with no need to add these separately

Analysis Note

Appearance (clearness): clear to turbid
Appearance (colour): yellowish-brown
pH-value (25 °C): 6.1 - 6.5
Recovery on test medium (Lactobacillus brevis ATCC 8287): ≥ 70 %
Recovery on test medium (Lactobacillus lindneri DSMZ 20690): ≥ 70 %
Recovery on test medium (Pediococcus damnosus ATCC 29358 (WDCM 00022)): ≥ 70 %
Recovery on test medium (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058)): ≥ 70 %
Recovery on test medium (Enterobacter aerogenes ATCC 13048): ≥ 70 %
Incubation: 1 up to 3 days; 30 °C; Lactobacillen and Pediococcus anaerobic; Saccharomyces and E.coli aerobic.

Other Notes

• The color of the prepared basal medium is clear and slightly brown and that of the medium with added beer is determined by the color of the beer.
• Do not use clumped or discolored medium.
• Store plates in the refrigerator and protect them from daylight.
• The shelf-life of prepared plates is approx. 1 week and 2 months for the medium dispensed into bottles when stored at 2-8°C.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3